Hollands Barca I, Miyares Cao C M, Santamarina Mijares A
Rev Cubana Med Trop. 1978 May-Aug;30(2):79-89.
The cholinesterase activity in Fasciola hepatica homogenates was studied through biological techniques. Results depict that the contractile stimulating action of a constant acetylcholine dose on the isolated rat duodenum is withdrawn when the agent is previously incubated at 37 degrees C during 30 minutes with different dilutions (20%, 40% and 80%) of Fasciola hepatica homogenates. The action is recovered when an anticholinesterase, as neostigmine is previously added to the homogenate. Since these effects are similar to those obtained when different dilutions of human blood serum with a high content of cholinesterase are led to act upon the acetylcholine dose, it is concluded that the acetylcholine inactivation induced by Fasciola hepatica homogenates results from the existence of such enzyme within this parasite.
通过生物学技术研究了肝片吸虫匀浆中的胆碱酯酶活性。结果表明，当一定剂量的乙酰胆碱与不同稀释度（20%、40%和80%）的肝片吸虫匀浆在37℃预孵育30分钟后作用于离体大鼠十二指肠时，其收缩刺激作用消失。当预先向匀浆中加入新斯的明等抗胆碱酯酶药物时，该作用恢复。由于这些效应与用高胆碱酯酶含量的不同稀释度人血清作用于乙酰胆碱剂量时获得的效应相似，因此得出结论，肝片吸虫匀浆诱导的乙酰胆碱失活是由于该寄生虫体内存在这种酶。
