Kumar Sunil, Anwer Razique, Azzi Arezki
Department of Microbiology, Kampala International University, Western Campus, Ishaka, Uganda.
Department of Pathology, College of Medicine, Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh, Saudi Arabia.
AIMS Microbiol. 2023 Feb 27;9(1):112-130. doi: 10.3934/microbiol.2023008. eCollection 2023.
The emergence and transmission of carbapenem-resistant (CRKP) have been recognized as a major public health concern. Here, we investigated the molecular epidemiology and its correlation with the mechanisms of resistance in CRKP isolates by compiling studies on the molecular epidemiology of CRKP strains worldwide. CRKP is increasing worldwide, with poorly characterized epidemiology in many parts of the world. Biofilm formation, high efflux pump gene expression, elevated rates of resistance, and the presence of different virulence factors in various clones of strains are important health concerns in clinical settings. A wide range of techniques has been implemented to study the global epidemiology of CRKP, such as conjugation assays, 16S-23S rDNA, string tests, capsular genotyping, multilocus sequence typing, whole-genome sequencing-based surveys, sequence-based PCR, and pulsed-field gel electrophoresis. There is an urgent need to conduct global epidemiological studies on multidrug-resistant infections of across all healthcare institutions worldwide to develop infection prevention and control strategies. In this review, we discuss different typing methods and resistance mechanisms to explore the epidemiology of pertaining to human infections.
耐碳青霉烯类肺炎克雷伯菌（CRKP）的出现和传播已被视为一个重大的公共卫生问题。在此，我们通过汇总全球范围内关于CRKP菌株分子流行病学的研究，调查了CRKP分离株的分子流行病学及其与耐药机制的相关性。CRKP在全球范围内呈上升趋势，世界许多地区的流行病学特征尚不明确。生物膜形成、高表达的外排泵基因、较高的耐药率以及不同菌株克隆中存在不同的毒力因子，是临床环境中的重要健康问题。已采用了多种技术来研究CRKP的全球流行病学，如接合试验、16S - 23S rDNA、串珠试验、荚膜基因分型、多位点序列分型、基于全基因组测序的调查、基于序列的PCR以及脉冲场凝胶电泳。迫切需要在全球所有医疗机构中开展关于多重耐药感染的全球流行病学研究，以制定感染预防和控制策略。在本综述中，我们讨论不同的分型方法和耐药机制，以探索与人类感染相关的流行病学。
