Department of Medical Microbiology, Faculty of Medicine, Hacettepe University, Ankara, Turkey.
Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Hacettepe University, Ankara, Turkey.
Microb Drug Resist. 2022 Mar;28(3):317-321. doi: 10.1089/mdr.2021.0114. Epub 2021 Dec 1.
The most common gram-negative pathogens in urinary tract infections are and . Therapy that is often empirical relies on local antibiotic resistance data, hence monitorization of antimicrobial resistance periodically in each hospital is a requirement. In this study, antibiotic susceptibility profiles of consecutive urinary isolates ( [ = 235] and [ = 56]) of adult patients collected between February 2018 and February 2019 from inpatients in Hacettepe University Hospital were assessed. Isolates resistant to fosfomycin (minimum inhibitory concentration >32 mg/L) were further investigated for the presence of , , and Fosfomycin susceptibility was determined by agar dilution method. Broth microdilution method was performed for amikacin, gentamicin, ceftazidime, ceftriaxone, meropenem, ciprofloxacin, tigecycline, trimethoprim/sulfamethoxazole (TMP/SMX), colistin, and piperacillin/tazobactam (PIP/TAZ). PCR method was employed to investigate , , , and . Existence of gene was confirmed by sequencing. Resistance rates to amikacin, gentamicin, ceftazidime, ceftriaxone, meropenem, ciprofloxacin, tigecycline, TMP/SMX, colistin, and PIP/TAZ were 2.7%, 18.5%, 25.4%, 33.0%, 3.4%, 45.4%, 2.4%, 43.6%, 6.2%, and 23.7%, respectively. Sixteen isolates (5.5%) were resistant to fosfomycin. Resistance was most frequently observed in ( = 9). gene was detected in one fosfomycin-resistant isolate. This isolate also carried and genes could not be detected in any of the isolates. In this study, we report for the first time the existence of in Turkey and its association with the gene. As a result of increasing bla producing isolates globally, increase in fosfomycin resistance may be expected in near future.
尿路感染最常见的革兰氏阴性病原体是 和 。通常经验性的治疗依赖于当地的抗生素耐药数据，因此定期监测每个医院的抗菌药物耐药性是一项要求。在这项研究中，评估了 2018 年 2 月至 2019 年 2 月期间从哈塞特佩大学医院住院患者中连续分离的成人尿液分离株（[=235]和[=56]）的抗生素敏感性谱。对磷霉素（最小抑菌浓度>32mg/L）耐药的分离株进一步研究了 、 、和 的存在情况。采用琼脂稀释法测定磷霉素的药敏性。采用肉汤微量稀释法测定阿米卡星、庆大霉素、头孢他啶、头孢曲松、美罗培南、环丙沙星、替加环素、复方磺胺甲噁唑（TMP/SMX）、黏菌素和哌拉西林/他唑巴坦（PIP/TAZ）的药敏性。采用 PCR 法检测 、 、 、 。通过测序证实 基因的存在。阿米卡星、庆大霉素、头孢他啶、头孢曲松、美罗培南、环丙沙星、替加环素、TMP/SMX、黏菌素和 PIP/TAZ 的耐药率分别为 2.7%、18.5%、25.4%、33.0%、3.4%、45.4%、2.4%、43.6%、6.2%和 23.7%。16 株（5.5%）分离株对磷霉素耐药。耐药性最常见于 （=9）。在一株对磷霉素耐药的 分离株中检测到 基因。该分离株还携带 和 基因，但在任何分离株中均未检测到 基因。在本研究中，我们首次在土耳其报告了 的存在及其与 基因的关联。由于全球 bla 产生的 分离株不断增加，预计在不久的将来，磷霉素的耐药性将会增加。
