School of Chemistry, University of Hyderabad, Gachibowli, Hyderabad, 500046, Telangana, India.
Department of Biomedical Engineering, Indian Institute of Technology, Kandi, 502284, Sangareddy, Telangana, India.
Chemistry. 2023 Jun 19;29(34):e202203796. doi: 10.1002/chem.202203796. Epub 2023 May 4.
The near-infrared (NIR) light-absorbing AgBiS nanoparticles can be excited by single-wavelength light, which is the primary characteristic of a photo responsive platform. Chemical synthesis of nanomaterials inevitably requires long-chain organic surfactants or polymers to stabilize them in the nano regime. These stabilizing molecules barricade the interaction of nanomaterials with biological cells. We have produced stabilizer-free (sf-AgBiS ) and polymer-coated (PEG-AgBiS ) nanoparticles; and assessed their NIR mediated anticancer and antibacterial activity to evaluate the effect of stabilizers. sf-AgBiS showed better antibacterial activity against Gram-positive Staphylococcus aureus (S. aureus) and displayed excellent cytotoxicity against HeLa cells and live 3-D tumour spheroids compared to PEG-AgBiS both in presence and absence of NIR radiation. The photothermal therapy (PTT) results illustrated the tumour ablation ability of sf-AgBiS , which converted light into heat effectively up to 53.3 °C under NIR irradiation. This work demonstrates the importance of synthesizing stabilizer-free nanoparticles to produce safe and highly active PTT agents.
近红外（NIR）吸光 AgBiS 纳米颗粒可以被单波长光激发，这是光响应平台的主要特征。纳米材料的化学合成不可避免地需要长链有机表面活性剂或聚合物来稳定它们在纳米级。这些稳定分子阻碍了纳米材料与生物细胞的相互作用。我们已经制备了无稳定剂（sf-AgBiS）和聚合物包覆（PEG-AgBiS）纳米颗粒，并评估了它们的近红外介导的抗癌和抗菌活性，以评估稳定剂的影响。sf-AgBiS 对革兰氏阳性金黄色葡萄球菌（S. aureus）显示出更好的抗菌活性，并且与 PEG-AgBiS 相比，在存在和不存在近红外辐射的情况下，对 HeLa 细胞和活的 3-D 肿瘤球体均显示出优异的细胞毒性。光热治疗（PTT）结果说明了 sf-AgBiS 的肿瘤消融能力，其在 NIR 照射下可将光有效地转化为热，最高可达 53.3°C。这项工作证明了合成无稳定剂纳米颗粒以生产安全且高活性 PTT 试剂的重要性。
