Department of Life Sciences, Pohang University of Science and Technology (POSTECH), Pohang, South Korea.
Methods Mol Biol. 2023;2651:167-177. doi: 10.1007/978-1-0716-3084-6_12.
Different from the canonical right-handed B-DNA, a left-handed Z-DNA forms an alternating syn- and anti-base conformations along the double-stranded helix under physiological conditions. Z-DNA structure plays a role in transcriptional regulation, chromatin remodeling, and genome stability. To understand the biological function of Z-DNA and map the genome-wide Z-DNA-forming sites (ZFSs), a ChIP-Seq strategy is applied, which is a combination of chromatin immunoprecipitation (ChIP) and high-throughput DNA sequencing analysis. Cross-linked chromatin is sheared and its fragments associated with Z-DNA-binding proteins are mapped onto the reference genome sequence. The global information of ZFSs positioning can provide a useful resource for better understanding of DNA structure-dependent biological mechanism.
与典型的右手 B-DNA 不同，在生理条件下，左手 Z-DNA 沿双链螺旋形成交替的顺式和反式碱基构象。Z-DNA 结构在转录调控、染色质重塑和基因组稳定性中发挥作用。为了了解 Z-DNA 的生物学功能并绘制全基因组 Z-DNA 形成位点 (ZFSs)，应用了一种 ChIP-Seq 策略，它是染色质免疫沉淀 (ChIP) 和高通量 DNA 测序分析的结合。交联的染色质被剪切，与 Z-DNA 结合蛋白相关的片段被映射到参考基因组序列上。ZFSs 定位的全局信息可为更好地理解 DNA 结构依赖性生物学机制提供有用的资源。
