人类β-珠蛋白基因簇内形成Z-DNA的位点。

Müller V, Takeya M, Brendel S, Wittig B, Rich A

Abteilung Molekularbiologie und Bioinformatik, Freie Universität Berlin, Germany.

Proc Natl Acad Sci U S A. 1996 Jan 23;93(2):780-4. doi: 10.1073/pnas.93.2.780.

Agarose-encapsulated, metabolically active, permeabilized nuclei from human hematopoietic cell lines were tested for Z-DNA formation in the beta-globin gene cluster. Biotinylated monoclonal antibodies against Z-DNA were diffused into the nuclei and cross-linked to DNA with a 10-ns laser exposure at 266 nm. Following digestion with restriction enzymes, fragments that had formed Z-DNA were isolated. Seventeen regions with Z-DNA sequence motifs in the 73-kb region were studied by PCR amplification, and five were found in the Z conformation.

对来自人类造血细胞系的琼脂糖包封、代谢活跃、通透化的细胞核进行测试，以检测β-珠蛋白基因簇中Z-DNA的形成。将抗Z-DNA的生物素化单克隆抗体扩散到细胞核中，并在266nm处以10纳秒的激光照射与DNA交联。用限制性内切酶消化后，分离出形成Z-DNA的片段。通过PCR扩增研究了73kb区域中具有Z-DNA序列基序的17个区域，发现其中5个处于Z构象。