Science for Life Laboratory, Department of Women's and Children's Health, Karolinska Institutet, 17165 Solna, Sweden.
Science for Life Laboratory, Division of Genome Biology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, 17165 Stockholm, Sweden.
Nano Lett. 2023 May 10;23(9):3701-3707. doi: 10.1021/acs.nanolett.2c04884. Epub 2023 Mar 9.
Speed is key during infectious disease outbreaks. It is essential, for example, to identify critical host binding factors to pathogens as fast as possible. The complexity of host plasma membrane is often a limiting factor hindering fast and accurate determination of host binding factors as well as high-throughput screening for neutralizing antimicrobial drug targets. Here, we describe a multiparametric and high-throughput platform tackling this bottleneck and enabling fast screens for host binding factors as well as new antiviral drug targets. The sensitivity and robustness of our platform were validated by blocking SARS-CoV-2 particles with nanobodies and IgGs from human serum samples.
速度是传染病爆发时的关键。例如，尽快识别病原体的关键宿主结合因子至关重要。宿主质膜的复杂性通常是一个限制因素，阻碍了宿主结合因子的快速准确确定以及中和抗菌药物靶点的高通量筛选。在这里，我们描述了一个多参数和高通量的平台，解决了这个瓶颈，使宿主结合因子的快速筛选以及新的抗病毒药物靶点成为可能。我们的平台的灵敏度和稳健性通过用来自人血清样本的纳米抗体和 IgG 阻断 SARS-CoV-2 颗粒得到了验证。
