使用静默替换法从人眼中分离黑视蛋白和视紫红质的方案。
Protocol for isolation of melanopsin and rhodopsin in the human eye using silent substitution.
机构信息
Centre for Vision and Eye Research, Queensland University of Technology (QUT), Brisbane, QLD 4059, Australia; School of Optometry and Vision Science, Queensland University of Technology (QUT), Brisbane, QLD 4059, Australia.
Centre for Vision and Eye Research, Queensland University of Technology (QUT), Brisbane, QLD 4059, Australia; School of Biomedical Sciences, Queensland University of Technology (QUT), Brisbane, QLD 4059, Australia; Queensland Eye Institute, Brisbane, QLD 4101, Australia.
出版信息
STAR Protoc. 2023 Mar 17;4(1):102126. doi: 10.1016/j.xpro.2023.102126. Epub 2023 Mar 7.
Melanopsin-mediated visual and non-visual functions are difficult to study in vivo. To isolate melanopsin responses, non-standard light stimulation instruments are required, with at least as many primaries as photoreceptor classes in the eye. In this protocol, we describe the physical light calibrations of the display instrumentation, control of stimulus artefacts, and correction of individual between-eye differences in human observers. The protocol achieves complete photoreceptor silent substitution in psychophysical, pupillometry, and electroretinographic experiments for probing melanopsin, rod, and cone function. For complete details on the use and execution of this protocol, please refer to Uprety et al. (2022)..
在体内研究褪黑素介导的视觉和非视觉功能很困难。为了分离褪黑素反应，需要使用非标准的光刺激仪器，其至少具有与眼睛中感光器种类一样多的原色。在本方案中，我们描述了显示仪器的物理光校准、刺激伪影的控制以及对人类观察者个体之间眼睛差异的校正。该方案可在探测褪黑素、视杆和视锥功能的心理物理学、瞳孔测量和视网膜电图实验中实现完全的感光器沉默替代。有关此方案使用和执行的完整详细信息，请参见 Uprety 等人（2022 年）。
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