猪脑中色胺的降解：一种新缩合产物的鉴定。

Degradation of tryptamine in pig brain: identification of a new condensation product.

作者信息

Susilo R, Höfle G, Rommelspacher H

机构信息

Institut für Neuropsychopharmakologie, Freie Universität Berlin, F.R. Germany.

出版信息

Biochem Biophys Res Commun. 1987 Nov 13;148(3):1045-52. doi: 10.1016/s0006-291x(87)80237-1.

DOI:10.1016/s0006-291x(87)80237-1

PMID:3689382

Abstract

Incubation of tryptamine with pig brain homogenate led to the formation of a product which is not identical with other known tryptamine metabolites. The same results were observed with rat brain tissue and bovine brain tissue. The compound has been isolated and identified by NMR spectroscopy, fast atom bombardment mass spectroscopy, and by chemical synthesis as a thiazolidine derivative, (4R)-2-(3-indolylmethyl)-1,3-thiazolidine-4-carboxylic acid. It is formed by a condensation reaction of indole-3-acetaldehyde generated enzymatically from tryptamine and of free L-cysteine present in the tissue. The compound inhibited monoamine oxidase (preferentially type A) and the neuronal gamma-aminobutyric acid uptake.

摘要

色胺与猪脑匀浆孵育会生成一种与其他已知色胺代谢产物不同的产物。在大鼠脑组织和牛脑组织中也观察到了相同的结果。该化合物已通过核磁共振光谱、快原子轰击质谱以及化学合成被分离并鉴定为噻唑烷衍生物，即(4R)-2-(3-吲哚基甲基)-1,3-噻唑烷-4-羧酸。它是由色胺酶促生成的吲哚-3-乙醛与组织中存在的游离L-半胱氨酸通过缩合反应形成的。该化合物抑制单胺氧化酶（优先抑制A型）和神经元γ-氨基丁酸摄取。