Tourasse Nicolas J, Jolley Keith A, Kolstø Anne-Brit, Økstad Ole Andreas
Department of Pharmacology and Pharmaceutical Biosciences, University of Oslo, Norway; University of Bordeaux, CNRS, INSERM, ARNA, UMR 5320, U1212, F-33000 Bordeaux, France.
Department of Biology, University of Oxford, UK.
Res Microbiol. 2023 Jul-Aug;174(6):104050. doi: 10.1016/j.resmic.2023.104050. Epub 2023 Mar 8.
Core genome multilocus sequence typing (cgMLST) employs a strategy where the set of orthologous genes common to all members of a group of organisms are used for phylogenetic analysis of the group members. The Bacillus cereus group consists of species with pathogenicity towards insect species as well as warm-blooded animals including humans. While B. cereus is an opportunistic pathogen linked to a range of human disease conditions, including emesis and diarrhoea, Bacillus thuringiensis is an entomopathogenic species with toxicity toward insect larvae, and therefore used as a biological pesticide worldwide. Bacillus anthracis is a classical obligate pathogen causing anthrax, an acute lethal condition in herbivores as well as humans, and which is endemic in many parts of the world. The group also includes a range of additional species, and B. cereus group bacteria have been subject to analysis with a wide variety of phylogenetic typing systems. Here we present, based on analyses of 173 complete genomes from B. cereus group species available in public databases, the identification of a set of 1568 core genes which were used to create a core genome multilocus typing scheme for the group which is implemented in the PubMLST system as an open online database freely available to the community. The new cgMLST system provides unprecedented resolution over existing phylogenetic analysis schemes covering the B. cereus group.
核心基因组多位点序列分型（cgMLST）采用一种策略，即利用一组生物体所有成员共有的直系同源基因集对该组成员进行系统发育分析。蜡样芽孢杆菌群包括对昆虫物种以及包括人类在内的温血动物具有致病性的物种。虽然蜡样芽孢杆菌是一种与包括呕吐和腹泻在内的一系列人类疾病状况相关的机会致病菌，但苏云金芽孢杆菌是一种对昆虫幼虫有毒性的昆虫病原菌，因此在全球范围内用作生物杀虫剂。炭疽芽孢杆菌是一种典型的专性病原菌，可导致炭疽病，这是一种在食草动物和人类中都会出现的急性致死性疾病，并且在世界许多地区都有流行。该菌群还包括一系列其他物种，并且蜡样芽孢杆菌群细菌已经通过多种系统发育分型系统进行了分析。在此，基于对公共数据库中173个蜡样芽孢杆菌群物种的完整基因组的分析，我们鉴定出一组1568个核心基因，这些基因被用于创建该菌群的核心基因组多位点分型方案，该方案在PubMLST系统中作为一个开放的在线数据库实施，供社区免费使用。新的cgMLST系统在覆盖蜡样芽孢杆菌群的现有系统发育分析方案上提供了前所未有的分辨率。
