Farina Donatella, Bianco Angelica, Manzulli Viviana, Castellana Stefano, Parisi Antonio, Caruso Marta, Fraccalvieri Rosa, Serrecchia Luigina, Rondinone Valeria, Pace Lorenzo, Fasanella Antonio, Vetritto Valerio, Difato Laura Maria, Cipolletta Dora, Iatarola Michela, Galante Domenico
Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Via Manfredonia 20, 71121 Foggia, Italy.
Antibiotics (Basel). 2024 Sep 20;13(9):898. doi: 10.3390/antibiotics13090898.
is a widespread environmental Gram-positive bacterium which is especially common in soil and dust. It produces two types of toxins that cause vomiting and diarrhea. At present, foodborne outbreaks due to group bacteria (especially sensu stricto) are rising, representing a serious problem in the agri-food supply chain. In this work, we analyzed 118 strains belonging to the group, isolated from several food sources, for which and antibiotic resistance assessments were performed. Many strains showed intermediate susceptibility to clindamycin, erythromycin, and tetracycline, suggesting an evolving acquisition of resistance against these antibiotics. Moreover, one strain showed intermediate resistance to meropenem, an antibiotic currently used to treat infections caused by . In addition to the phenotypic antimicrobial resistance profile, all strains were screened for the presence/absence of antimicrobial genes via whole-genome sequencing. There was inconsistency between the and analyses, such as in the case of vancomycin, for which different isolates harbored resistance genes but, phenotypically, the same strains were sensitive. This would suggest that antibiotic resistance is a complex phenomenon due to a variety of genetic, epigenetic, and biochemical mechanisms.
是一种广泛存在的环境革兰氏阳性菌，在土壤和灰尘中尤为常见。它产生两种导致呕吐和腹泻的毒素。目前，由该菌属细菌（尤其是狭义的该菌）引起的食源性疾病暴发呈上升趋势，这在农业食品供应链中是一个严重问题。在这项工作中，我们分析了从多种食物来源分离出的118株该菌属菌株，并对其进行了抗生素敏感性评估。许多菌株对克林霉素、红霉素和四环素表现出中度敏感性，这表明对这些抗生素的耐药性在不断演变。此外，一株菌株对美罗培南表现出中度耐药，美罗培南是目前用于治疗由该菌引起的感染的一种抗生素。除了表型抗菌耐药谱外，还通过全基因组测序对所有菌株进行了抗菌基因存在与否的筛选。在基因分析和表型分析之间存在不一致情况，例如在万古霉素的案例中，不同分离株携带耐药基因，但在表型上，相同菌株却是敏感的。这表明抗生素耐药性是一种由于多种遗传、表观遗传和生化机制导致的复杂现象。
