Sterri S H, Fonnum F
Norwegian Defence Research Establishment, Division for Environmental Toxicology, Kjeller.
Biochem Pharmacol. 1987 Nov 15;36(22):3937-42. doi: 10.1016/0006-2952(87)90461-8.
The carboxylesterase activity in both plasma and liver of guinea-pig were separated into three main peaks by chromatofocusing. Two of the three plasma enzymes were retained by affinity chromatography on Affi-Gel Blue (100-200 mesh). Isoelectric points determined by chromatofocusing or isoelectrofocusing were pI 6.1, pI 5.2 and pI 4.0 for the plasma enzymes, and pI 5.7, pI 5.2 and pI 4.5 for the liver enzymes. The effect of selective esterase inhibitors, soman, physostigmine (cholinesterase inhibitor) and bis-4-nitrophenyl phosphate (carboxylesterase inhibitor), suggested that the three enzymes in both tissues may be regarded as carboxylesterases. However, the pI 5.7 carboxylesterase was partially inhibited by physostigmine, and the pI 4.5 carboxylesterase was almost not affected by bis-4-nitrophenyl phosphate. The ratio between the activities towards 4-nitrophenyl butyrate and methyl butyrate differed among the carboxylesterases in both tissues. All three carboxylesterases in plasma were partially reactivated by diacetylmonoxime after soman inhibition in vitro, but to a different extent. The soman inhibited liver carboxylesterases were not reactivated by diacetylmonoxime.
通过层析聚焦法，豚鼠血浆和肝脏中的羧酸酯酶活性被分离为三个主要峰。三种血浆酶中的两种可通过在Affi-Gel Blue（100 - 200目）上的亲和层析保留。通过层析聚焦法或等电聚焦法测定的血浆酶的等电点分别为pI 6.1、pI 5.2和pI 4.0，肝脏酶的等电点分别为pI 5.7、pI 5.2和pI 4.5。选择性酯酶抑制剂梭曼、毒扁豆碱（胆碱酯酶抑制剂）和磷酸双-4-硝基苯酯（羧酸酯酶抑制剂）的作用表明，两种组织中的三种酶都可被视为羧酸酯酶。然而，pI 5.7的羧酸酯酶被毒扁豆碱部分抑制，pI 4.5的羧酸酯酶几乎不受磷酸双-4-硝基苯酯的影响。两种组织中羧酸酯酶对丁酸4-硝基苯酯和丁酸甲酯的活性比值不同。在体外梭曼抑制后，血浆中的所有三种羧酸酯酶都可被双乙酰单肟部分复活，但复活程度不同。梭曼抑制的肝脏羧酸酯酶不能被双乙酰单肟复活。
