Institute of Zoology, University of Belgrade-Faculty of Biology, Studentski Trg 16, 11000, Belgrade, Serbia.
Faculty of Technology Zvornik, University of East Sarajevo, Karakaj 34a, 75400, Zvornik, Republic of Srpska, Bosnia and Herzegovina.
J Comp Physiol B. 2023 Jun;193(3):249-260. doi: 10.1007/s00360-023-01481-w. Epub 2023 Mar 9.
The activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), and glutathione S-transferase (GST), as well as the concentrations of sulfhydryl (SH) groups and glutathione (GSH) were analyzed in five age classes of the Mediterranean centipede Scolopendra cingulata as follows: embryo, adolescens, maturus junior, maturus, and maturus senior. The data obtained showed the presence of SOD, CAT, GSH-Px, GR, GST, and SH groups in embryos. The transition from embryo to adolescens was accompanied by an increase in the activities of all studied enzymes, in response to the increased production of ROS due to the increased metabolic activity of the centipede associated with growth and development. Our results show that trends in antioxidant enzyme (AOE) activities were not uniform among adult age classes, suggesting that maturus junior, maturus, and maturus senior differentially respond and/or have different susceptibility to ROS. On the other hand, GSH concentration in embryos was undetectable, highest in adolescens and decreased in the latter part of life. Pearson correlation analysis in embryos showed that the activities of the AOEs were strongly and positively correlated with each other but negatively correlated with GSH and SH groups. At later age classes, SOD, CAT, GSH-Px, GR, GSH, and SH groups were no longer significantly correlated with GST. In the discriminant analysis, the variables that separated the age classes were GR, GST, SH groups, and body length. Body length was directly related to the age of individuals, clearly indicating that development/aging affects the regulation of antioxidant defense in this species.
对 5 个年龄组（胚胎、青春期、幼体成熟、成体成熟和老年）的地中海蜈蚣 Scolopendra cingulata 中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）、谷胱甘肽还原酶（GR）和谷胱甘肽 S-转移酶（GST）的活性以及巯基（SH）基团和谷胱甘肽（GSH）的浓度进行了分析。胚胎中存在 SOD、CAT、GSH-Px、GR、GST 和 SH 基团。从胚胎到青春期的转变伴随着所有研究酶活性的增加，这是由于蜈蚣代谢活动增加导致活性氧（ROS）产生增加所致，而代谢活动的增加与生长和发育有关。我们的研究结果表明，成年年龄组之间抗氧化酶（AOE）活性的趋势并不一致，这表明幼体成熟、成体成熟和老年对 ROS 的反应不同，或者对 ROS 的敏感性不同。另一方面，胚胎中 GSH 的浓度不可检测，青春期最高，生命后期降低。胚胎中的 Pearson 相关分析表明，AOE 的活性彼此之间呈强正相关，但与 GSH 和 SH 基团呈负相关。在后期年龄组中，SOD、CAT、GSH-Px、GR、GSH 和 SH 组与 GST 不再显著相关。在判别分析中，区分年龄组的变量是 GR、GST、SH 基团和身体长度。身体长度与个体年龄直接相关，这清楚地表明发育/衰老会影响该物种抗氧化防御的调节。
