Department of Clinical Laboratory, Huaihe Hospital of Henan University, 115 Ximen Street, Kaifeng, Henan, China.
Department of Nephrology, First Affiliated Hospital of Henan University, Kaifeng, China.
Curr Microbiol. 2023 Mar 9;80(4):132. doi: 10.1007/s00284-023-03219-x.
An association between type 2 diabetes mellitus (T2DM) and gut microbiota is well established, but the results of related studies are inconsistent. The purpose of this investigation is to elucidate the characteristics of the gut microbiota in T2DM and non-diabetic subjects. Forty-five subjects were recruited for this study, including 29 T2DM patients and 16 non-diabetic subjects. Biochemical parameters, including body mass index (BMI), fasting plasma glucose (FPG), serum total cholesterol (TC), triglycerides (TG), high-density lipoprotein (HDL), and hemoglobin A1c (HbA1c), were analyzed and correlated with the gut microbiota. Bacterial community composition and diversity were detected in fecal samples using direct smear, sequencing, and real-time polymerase chain reaction (PCR). In this study, it was observed that indicators such as BMI, FPG, HbA1c, TC, and TG in T2DM patients were on the rise, concurrent with dysbiosis of the microbiota. We observed an increase in Enterococci and a decrease in Bacteroides, Bifidobacteria, and Lactobacilli in patients with T2DM. Meanwhile, total short-chain fatty acids (SCFAs) and D-lactate concentrations were decreased in the T2DM group. In addition, FPG was positively correlated with Enterococcus and negatively correlated with Bifidobacteria, Bacteroides, and Lactobacilli. This study reveals that microbiota dysbiosis is associated with disease severity in patients with T2DM. The limitation of this study is that only common bacteria were noted in this study, and more in-depth related studies are urgently needed.
2 型糖尿病（T2DM）与肠道微生物群之间存在关联，但相关研究结果并不一致。本研究旨在阐明 T2DM 患者和非糖尿病患者肠道微生物群的特征。本研究纳入了 45 名受试者，包括 29 名 T2DM 患者和 16 名非糖尿病患者。分析了受试者的生化参数，包括体重指数（BMI）、空腹血糖（FPG）、血清总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白（HDL）和糖化血红蛋白（HbA1c），并与肠道微生物群进行了相关性分析。采用直接涂片、测序和实时聚合酶链反应（PCR）检测粪便样本中的细菌群落组成和多样性。本研究观察到，T2DM 患者的 BMI、FPG、HbA1c、TC 和 TG 等指标呈上升趋势，同时伴有微生物群失调。我们观察到 T2DM 患者肠球菌增加，拟杆菌、双歧杆菌和乳酸杆菌减少。同时，T2DM 组总短链脂肪酸（SCFA）和 D-乳酸浓度降低。此外，FPG 与肠球菌呈正相关，与双歧杆菌、拟杆菌和乳酸杆菌呈负相关。本研究表明，肠道微生物群失调与 T2DM 患者的疾病严重程度有关。本研究的局限性在于仅观察到常见细菌，因此需要进行更深入的相关研究。
