Ratushnyak M G, Zhirnik A S, Smirnova O D, Semochkina Yu P, Parfenova A A, Goryunov K V, Silachev D N, Moskaleva E Yu
National Research Center "Kurchatov Institute", Moscow, Russia.
V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.
Bull Exp Biol Med. 2023 Feb;174(4):571-577. doi: 10.1007/s10517-023-05749-7. Epub 2023 Mar 10.
We studied the effect of intranasal administration of neural stem cell (NSC)-derived exosomes on behavior and cognitive functions of mice in the late period after head irradiation in a dose of 8 Gy. The used exosomes had specific markers (CD9/CD63, 99.5%; TSG101, 98.4%) and mean size 105.7±8.8 nm according to dynamic light scattering data and 119.0±12.4 nm according to nanoparticle tracking analysis (NTA). Exosome suspension (2×10 particles/ml according to NTA measurements) was administered intranasally for 4 weeks starting from 48 h after irradiation in a volume of 5 μl/nostril (2×10 exosomes/mouse). It was shown that intranasal administration of mouse NSC-derived exosomes prevented delayed radiation-induced behavioral changes and recognition memory impairments in mice after head irradiation.
我们研究了经鼻给予神经干细胞（NSC）来源的外泌体对8 Gy头部照射后晚期小鼠行为和认知功能的影响。根据动态光散射数据，所用外泌体具有特异性标志物（CD9/CD63，99.5%；TSG101，98.4%），平均大小为105.7±8.8 nm，根据纳米颗粒跟踪分析（NTA）数据，平均大小为119.0±12.4 nm。从照射后48小时开始，以5 μl/鼻孔（2×10个外泌体/小鼠）的体积经鼻给予外泌体悬浮液（根据NTA测量为2×10个颗粒/毫升），持续4周。结果表明，经鼻给予小鼠NSC来源的外泌体可预防头部照射后小鼠延迟性辐射诱导的行为变化和识别记忆障碍。
