National Research Center "Kurchatov Institute", Moscow, Russia.
Bull Exp Biol Med. 2022 Aug;173(4):544-552. doi: 10.1007/s10517-022-05587-z. Epub 2022 Sep 5.
The death of neural stem cells in the hippocampus during radiation therapy of brain tumors leads to neurogenesis impairment and the development of cognitive dysfunctions at delayed terms after irradiation. Exosomes secreted by stem cells can provide a protective effect on neural stem cells. We isolated and characterized exosomes from the medium conditioned by neural stem cells and mesenchymal stem cells from mouse adipose tissue and studied their efficiency in protecting irradiated neural stem cells. According to dynamic light scattering data, the exosome size varied from 44 to 68 nm for neural stem cells, and from 80 to 130 nm for mesenchymal stem cells. All exosomes carried markers CD9, CD63, and TSG101. The survival rate and clonogenic activity of neural stem cells irradiated in a dose of 1 Gy was found to increase after culturing in the presence of stem cell exosome preparations.
放射治疗脑瘤时，海马区神经干细胞死亡会导致神经发生障碍，并在照射后延迟期出现认知功能障碍。干细胞分泌的外泌体对神经干细胞具有保护作用。我们从来自于小鼠脂肪组织的神经干细胞和间充质干细胞的培养基中分离并鉴定了外泌体，并研究了它们对受照射神经干细胞的保护效率。根据动态光散射数据，神经干细胞来源的外泌体大小为 44 至 68nm，间充质干细胞来源的外泌体大小为 80 至 130nm。所有外泌体均携带 CD9、CD63 和 TSG101 标志物。在 1Gy 剂量下照射的神经干细胞在存在干细胞外泌体制剂的情况下培养后，其存活率和克隆形成活性增加。
