Du Pan, Chen Xue, Chen Yang, Li Jin, Lu Yichi, Li Xiaoxiao, Hu Kai, Chen Junfeng, Lv Guozhong
Jiangnan University Wuxi School of Medicine, Wuxi, Jiangsu, 214122, China.
Nanjing University of Chinese Medicine, Nanjing, 210000, China.
Heliyon. 2023 Feb 15;9(3):e13506. doi: 10.1016/j.heliyon.2023.e13506. eCollection 2023 Mar.
In this study, electrospun nanofibers (NFs) used in trauma dressings were prepared using silk fibroin (SF) and gelatin (GT) as materials and highly volatile formic acid as the solvent, with three different concentrations of propolis extracts (EP), which were loaded through a simple process. The resulting samples were characterized by surface morphology, scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), contact angle meter, water absorption, degradation rate, and mechanical property tests. The incorporation of propolis improved its antibacterial properties against , and , compared to those of the silk gelatin nanofiber material (SF/GT) alone. In vitro biocompatibility assays showed that SF/GT-1%EP had good cytocompatibility and hemocompatibility. In addition, it can also significantly promote the migration of L929 cells. SF/GT-1%EP was applied to a mouse model of full thickness skin defects, and it was found to significantly promote wound healing. These results indicate that the SF/GT-EP nanofiber material has good biocompatibility, migrating-promoting capability, antibacterial properties, and healing-promoting ability, providing a new idea for the treatment of full thickness skin defects.
在本研究中，以丝素蛋白（SF）和明胶（GT）为材料，高挥发性甲酸为溶剂，制备了用于创伤敷料的电纺纳米纤维（NFs），并通过简单工艺负载了三种不同浓度的蜂胶提取物（EP）。通过表面形貌、扫描电子显微镜（SEM）、傅里叶变换红外光谱（FTIR）、接触角测量仪、吸水率、降解率和力学性能测试对所得样品进行了表征。与单独的丝胶纳米纤维材料（SF/GT）相比，蜂胶的加入改善了其对金黄色葡萄球菌、大肠杆菌和铜绿假单胞菌的抗菌性能。体外生物相容性试验表明，SF/GT-1%EP具有良好的细胞相容性和血液相容性。此外，它还能显著促进L929细胞的迁移。将SF/GT-1%EP应用于全层皮肤缺损小鼠模型，发现其能显著促进伤口愈合。这些结果表明，SF/GT-EP纳米纤维材料具有良好的生物相容性、促迁移能力、抗菌性能和促愈合能力，为全层皮肤缺损的治疗提供了新思路。
