Zhang Qingyi, Li Yue, Cao Kun, Xu Hongwei, Zhou Xiaofu
Key Laboratory of Plant Resource Science and Green Production of Jilin Province, Jilin Normal University, Siping, China.
Front Plant Sci. 2023 Feb 21;14:1146663. doi: 10.3389/fpls.2023.1146663. eCollection 2023.
Cold stress is a global common problem that significantly limits plant development and geographical distribution. Plants respond to low temperature stress by evolving interrelated regulatory pathways to respond and adapt to their environment in a timely manner. Pall. () is a perennial evergreen dwarf shrub used for adornment and medicine that thrives in the Changbai Mountains at high elevations and subfreezing conditions.
In this study, a comprehensive investigation of cold tolerance (4°C, 12h) in leaves under cold using physiological combined with transcriptomic and proteomic approaches.
There were 12,261 differentially expressed genes (DEGs) and 360 differentially expressed proteins (DEPs) in the low temperature (LT) and normal treatment (Control). Integrated transcriptomic and proteomic analyses showed that MAPK cascade, ABA biosynthesis and signaling, plant-pathogen interaction, linoleic acid metabolism and glycerophospholipid metabolism were significantly enriched in response to cold stress of leaves.
We analyzed the involvement of ABA biosynthesis and signaling, MAPK cascade, and Ca signaling, that may jointly respond to stomatal closure, chlorophyll degradation, and ROS homeostasis under low temperature stress. These results propose an integrated regulatory network of ABA, MAPK cascade and Ca signaling comodulating the cold stress in , which will provide some insights to elucidate the molecular mechanisms of cold tolerance in plants.
冷胁迫是一个全球性的普遍问题，严重限制了植物的生长发育和地理分布。植物通过进化相互关联的调控途径来及时响应和适应环境，从而应对低温胁迫。朝鲜冷杉（Pall.）是一种多年生常绿矮灌木，可用于装饰和药用，在高海拔和亚冰点条件的长白山生长旺盛。
在本研究中，采用生理结合转录组学和蛋白质组学方法，对朝鲜冷杉叶片在4°C、12小时冷胁迫下的耐寒性进行了全面研究。
在低温（LT）和正常处理（对照）下，有12261个差异表达基因（DEGs）和360个差异表达蛋白（DEPs）。综合转录组学和蛋白质组学分析表明，丝裂原活化蛋白激酶（MAPK）级联、脱落酸（ABA）生物合成和信号传导、植物-病原体相互作用、亚油酸代谢和甘油磷脂代谢在朝鲜冷杉叶片对冷胁迫的响应中显著富集。
我们分析了ABA生物合成和信号传导、MAPK级联和钙信号传导的参与情况，它们可能在低温胁迫下共同响应气孔关闭、叶绿素降解和活性氧（ROS）稳态。这些结果提出了一个由ABA、MAPK级联和钙信号传导共同调节朝鲜冷杉冷胁迫的综合调控网络，这将为阐明植物耐寒性的分子机制提供一些见解。
