葛根多糖及其衍生物的制备、结构分析与抗氧化活性研究。

Preparation, Structural Analysis and Antioxidant Activity of Polysaccharides and Their Derivatives from Pueraria lobata.

机构信息

Key Laboratory of Carbohydrate Science and Engineering, Chongqing Normal University, Chongqing, 401331, China.

出版信息

Chem Biodivers. 2023 Apr;20(4):e202201253. doi: 10.1002/cbdv.202201253. Epub 2023 Mar 27.

DOI:10.1002/cbdv.202201253

Abstract

Pueraria lobata polysaccharides (PLPs) were obtained by hot water extraction using Pueraria lobata as raw material. Structural analysis revealed that PLPs may have a repetitive backbone units of →4) -α-D-Glcp (1→4-α-D-Glcp (1→. Phosphorylated Pueraria lobata polysaccharides (P-PLPs), carboxymethylated Pueraria lobata polysaccharides (CM-PLPs) and acetylated Pueraria lobata polysaccharides (Ac-PLPs) were obtained by chemical modifications of PLPs, respectively. The physicochemical properties and antioxidant activities of these four Pueraria lobata polysaccharides were studied in comparison. In particular, the clearance rate of P-PLPs exceeded 80 %, and was expected to achieve the same effect as V . The results showed that the effects of different chemical modifications on the antioxidant activity of PLPs varied greatly.

摘要

葛根多糖（PLPs）以葛根为原料，采用热水提取法获得。结构分析表明，PLPs 可能具有重复的→4)-α-D-Glcp（1→4-α-D-Glcp（1→的主链单元。通过对 PLPs 进行化学修饰，分别得到了磷酸化葛根多糖（P-PLPs）、羧甲基化葛根多糖（CM-PLPs）和乙酰化葛根多糖（Ac-PLPs）。对这四种葛根多糖的理化性质和抗氧化活性进行了比较研究。特别是，P-PLPs 的清除率超过 80%，有望达到与 V 相同的效果。结果表明，不同化学修饰对 PLPs 抗氧化活性的影响差异很大。