Qian Kai, Tan Ting, Ouyang Hui, Yang Shi-Lin, Zhu Wei-Feng, Liu Rong-Hua, Wen Quan, Feng Yu-Lin
Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, Jiangxi, PR China.
Food Funct. 2020 Aug 1;11(8):7104-7114. doi: 10.1039/d0fo01234c. Epub 2020 Aug 3.
A water-soluble neutral homopolysaccharide (PLP-1) was obtained from the roots of Pueraria lobata by DEAE cellulose and Sephadex G-200 gel chromatography purification. The average molecular weight of PLP-1 was 16.2 kDa. Monosaccharide composition analysis showed that PLP-1 was composed of glucose as a glucan. The structure of PLP-1 was characterized on the basis of extensive physical and chemical analysis, which indicated that the backbone of PLP-1 was mainly composed of →3)-α-d-Glcp(1→ and →4)-β-d-Glcp(1→ with a molar ratio of 7.0 : 1.0. Moreover, the hypoglycemic activity of PLP-1 was investigated by palmitic acid and high glucose induced insulin resistant HepG2 cells. The results elucidated that PLP-1 could decrease the glucose concentration by up-regulating the expression of PI3K and AKT, and down-regulating the expression of FoxO1, PCK2, and G6Pase in insulin resistant cells. Therefore, PLP-1 could serve as a dietary supplement to ameliorate insulin resistance for diabetic patients.
通过DEAE纤维素和Sephadex G - 200凝胶色谱纯化从葛根根中获得了一种水溶性中性同多糖（PLP - 1）。PLP - 1的平均分子量为16.2 kDa。单糖组成分析表明PLP - 1作为一种葡聚糖由葡萄糖组成。基于广泛的物理和化学分析对PLP - 1的结构进行了表征，结果表明PLP - 1的主链主要由→3)-α - d - Glcp(1→和→4)-β - d - Glcp(1→组成，摩尔比为7.0∶1.0。此外，通过棕榈酸和高糖诱导胰岛素抵抗的HepG2细胞研究了PLP - 1的降血糖活性。结果表明，PLP - 1可通过上调PI3K和AKT的表达以及下调胰岛素抵抗细胞中FoxO1、PCK2和G6Pase的表达来降低葡萄糖浓度。因此，PLP - 1可作为一种膳食补充剂来改善糖尿病患者的胰岛素抵抗。
