Key Laboratory of Veterinary Chemical Drugs and Pharmaceutics, Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China.
Int J Mol Sci. 2023 Mar 26;24(7):6247. doi: 10.3390/ijms24076247.
A novel water-soluble acidic heteropolysaccharide, called PPL-1, was purified from . PPL-1 had an average molecular weight of 35 Kad, and it was composed of glucose, arabinose, galactose and galacturonic acid (6.3:0.8:0.8:2.1). In accordance with methylation and nuclear magnetic resonance analyses, PPL-1 primarily consisted of (1→2)-linked α-Araf, (1→4)-linked α-Glcp, (1→)-linked β-Glcp, (1→6)-linked α-Glcp, (1→3,6)-linked α-Galp, (1→)-linked β-GalpA and (1→4)-linked α-GalpA. In terms of bioactivities, PPL-1 exhibited remarkable scavenging ability towards DPPH (1,1-Diphenyl-2-picrylhydrazyl) radicals and moderate activity by enhancing the proliferation rate of RAW 264.7 cells by approximately 30% along with the secretion of NO. This work demonstrates that PPL-1 can be a potential source of immunoenhancers and antioxidants.
从 中提取出一种新型水溶性酸性杂多糖，命名为 PPL-1。PPL-1 的平均分子量为 35kDa，由葡萄糖、阿拉伯糖、半乳糖和半乳糖醛酸（6.3:0.8:0.8:2.1）组成。根据甲基化和核磁共振分析，PPL-1 主要由（1→2）-连接的α-Araf、（1→4）-连接的α-Glcp、（1→）-连接的β-Glcp、（1→6）-连接的α-Glcp、（1→3,6）-连接的α-Galp、（1→）-连接的β-GalpA 和（1→4）-连接的α-GalpA 组成。在生物活性方面，PPL-1 对 DPPH（1,1-二苯基-2-苦基肼）自由基具有显著的清除能力，并且能够将 RAW 264.7 细胞的增殖率提高约 30%，同时促进 NO 的分泌。这项工作表明，PPL-1 可能是免疫增强剂和抗氧化剂的潜在来源。
