Halliday H, Robertson B, Nilsson R, Rigaut J P, Grossmann G
Department of Neonatology, Royal Maternity Hospital, Belfast, Northern Ireland.
Br J Exp Pathol. 1987 Oct;68(5):727-32.
Automated image analysis of histological lung sections was used to compare the efficacy of an artificial surfactant (dipalmitoylphosphatidylcholine + high-density lipoprotein, 10:1) and a natural surfactant (the phospholipid fraction of porcine surfactant, isolated by liquid-gel chromatography in ventilated immature newborn rabbits delivered after 27 days' gestation. Tidal volumes were significantly improved in each group treated with surfactant when compared with controls, but natural surfactant-treated rabbits had significantly higher tidal volumes than those receiving artificial surfactant. There were no statistically significant differences in alveolar expansion between the artificial surfactant group and the controls, but alveolar volume density and a shape factor (assessing the 'circularity' of terminal airspaces) were significantly higher in animals receiving natural surfactant. These animals also had a lower coefficient of variation of alveolar volume density and a lower alveolar average integral mean surface curvature, indicating a uniform pattern of alveoli with a smooth profile. We conclude that automated image analysis is useful for the quantitation of alveolar expansion patterns in immature neonatal lungs and that natural surfactant is superior to the artificial surfactant tested in the present study.
采用组织学肺切片的自动图像分析技术，比较一种人工表面活性剂（二棕榈酰磷脂酰胆碱+高密度脂蛋白，比例为10:1）和一种天然表面活性剂（通过液-凝胶色谱法分离的猪表面活性剂的磷脂部分）对妊娠27天后出生的未成熟新生兔进行机械通气时的效果。与对照组相比，每组接受表面活性剂治疗的潮气量均显著改善，但接受天然表面活性剂治疗的兔子的潮气量显著高于接受人工表面活性剂治疗的兔子。人工表面活性剂组与对照组之间的肺泡扩张无统计学显著差异，但接受天然表面活性剂治疗的动物的肺泡容积密度和一个形状因子（评估终末气腔的“圆形度”）显著更高。这些动物的肺泡容积密度变异系数也更低，肺泡平均积分平均表面曲率也更低，表明肺泡形态均匀且轮廓光滑。我们得出结论，自动图像分析对于定量未成熟新生肺的肺泡扩张模式很有用，并且天然表面活性剂优于本研究中测试的人工表面活性剂。
