Khaleghi Maryam, Haghi Fakhri, Gholami Mina, Hourfar Hamdam, Shahi Farshad, Mir Mousavi Zekoloujeh Ali, Aliakbari Farhang, Ahmadi Ebrahim, Morshedi Dina
Bioprocess Engineering Department, Institute of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology, Shahrak-E Pajoohesh, km 15 Tehran-Karaj Highway, 14965/161, Tehran, Iran.
Department of Microbiology, School of Medicine, Zanjan University of Medical Sciences, 45139-56111, Zanjan, IR, Iran.
AMB Express. 2023 Mar 10;13(1):29. doi: 10.1186/s13568-023-01533-y.
High risk of acute morbidities and even mortality from expanding the antibiotics resistant infectious wounds force indefinite efforts for development of high performance wound-healing materials. Herein, we design a procedure to fabricate a hyaluronic acid (HA)-based hydrogel to conjugate curcumin (Gel-H.P.Cur). The highlight of this work is to provide a favorite condition for capturing curcumin while protecting its structure and intensifying its activities because of the synchronization with HA. Accordingly, HA as a major component of dermis with a critical role in establishing skin health, could fortify the wound healing property as well as antibacterial activity of the hydrogel. Gel-H.P.Cur showed antibacterial properties against Pseudomonas aeruginosa (P. aeruginosa), which were examined by bactericidal efficiency, disk diffusion, anti-biofilm, and pyocyanin production assays. The effects of Gel-H.P.Cur on the inhibition of quorum sensing (QS) regulatory genes that contribute to expanding bacteria in the injured place was also significant. In addition, Gel-H.P.Cur showed high potential to heal the cutaneous wounds on the mouse excisional wound model with repairing histopathological damages rapidly and without scar. Taken together, the results strongly support Gel-H.P.Cur as a multipotent biomaterial for medical applications regarding the treatment of chronic, infected, and dehiscent wounds.
抗生素耐药性感染伤口不断增加，导致急性发病甚至死亡的风险很高，这促使人们不断努力研发高性能的伤口愈合材料。在此，我们设计了一种制备基于透明质酸（HA）的水凝胶以共轭姜黄素（Gel-H.P.Cur）的方法。这项工作的亮点在于，由于与HA同步，为捕获姜黄素提供了有利条件，同时保护其结构并增强其活性。因此，HA作为真皮的主要成分，在维持皮肤健康方面起着关键作用，可以增强水凝胶的伤口愈合性能以及抗菌活性。通过杀菌效率、纸片扩散法、抗生物膜和绿脓菌素产生试验检测，Gel-H.P.Cur对铜绿假单胞菌（P. aeruginosa）具有抗菌性能。Gel-H.P.Cur对抑制群体感应（QS）调节基因的作用也很显著，这些基因有助于受伤部位细菌的扩散。此外，在小鼠切除伤口模型上，Gel-H.P.Cur显示出治愈皮肤伤口的巨大潜力，能迅速修复组织病理学损伤且不留疤痕。综上所述，这些结果有力地支持了Gel-H.P.Cur作为一种多能生物材料在治疗慢性、感染性和裂开性伤口的医学应用中的地位。
