Thakur Mony, Kumar Punit, Rajput Deepanshi, Yadav Vinod, Dhaka Namrata, Shukla Rishikesh, Kumar Dubey Kashyap
Department of Biotechnology, Central University of Haryana, Mahendergarh 123031, India.
Department of Microbiology, Central University of Haryana, Mahendergarh 123031, India.
Bioresour Technol. 2023 May;376:128836. doi: 10.1016/j.biortech.2023.128836. Epub 2023 Mar 9.
Streptomyces genera serve as adaptable cell factories for secondary metabolites with various and distinctive chemical structures that are relevant to the pharmaceutical industry. Streptomyces' complex life cycle necessitated a variety of tactics to enhance metabolite production. Identification of metabolic pathways, secondary metabolite clusters, and their controls have all been accomplished using genomic methods. Besides this, bioprocess parameters were also optimized for the regulation of morphology. Kinase families were identified as key checkpoints in the metabolic manipulation (DivIVA, Scy, FilP, matAB, and AfsK) and morphology engineering of Streptomyces. This review illustrates the role of different physiological variables during fermentation in the bioeconomy coupled with genome-based molecular characterization of biomolecules responsible for secondary metabolite production at different developmental stages of the Streptomyces life cycle.
链霉菌属是适应性强的细胞工厂，可用于生产具有各种独特化学结构且与制药行业相关的次级代谢产物。链霉菌复杂的生命周期需要多种策略来提高代谢产物产量。利用基因组方法已完成了代谢途径、次级代谢产物簇及其调控的鉴定。除此之外，还对生物过程参数进行了优化以调控形态。激酶家族被确定为链霉菌代谢调控（DivIVA、Scy、FilP、matAB和AfsK）和形态工程中的关键控制点。本综述阐述了发酵过程中不同生理变量在生物经济中的作用，以及在链霉菌生命周期不同发育阶段负责次级代谢产物产生的生物分子基于基因组的分子特征。
