Chang Ting-Ting, Bian Lu-Yao, Zhang Chong
Laboratory of Food Industrial Enzyme Technology, College of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
Biotechnol Lett. 2025 May 17;47(3):57. doi: 10.1007/s10529-025-03598-0.
Transglutaminase (TGase) is widely utilized in food, textile, and medical applications due to its superior capacity to facilitate protein cross-linking. The mechanisms driving high TGase production in Streptomyces mobaraensis require further exploration. "Microparticle-Enhanced Cultivation" (MPEC), has been shown to significantly influence the morphology of filamentous microorganisms, thereby impacting secondary metabolism.
In the study, CaCO and talc were found to increase the TGase activity of HVCP-Sm1 from 15 U/mL to 29.05 U/mL and 31 U/mL respectively. Moreover, CaCO and talc were observed to reduce the diameters of hyphal particles and elongated hyphae while promoting pro-TGase activation. This research expands the understanding of the role of MPEC in the morphology of filamentous microorganisms and its effects on secondary metabolism.
The synergistic effects of group sensing, energy metabolism, and the optimization of mycelial morphology enhanced the secondary metabolism of HVCP-Sm1 and increased TGase enzymatic activity.
谷氨酰胺转胺酶（TGase）因其在促进蛋白质交联方面的卓越能力而被广泛应用于食品、纺织和医学领域。莫氏链霉菌中驱动高TGase产量的机制需要进一步探索。“微粒增强培养”（MPEC）已被证明会显著影响丝状微生物的形态，从而影响次级代谢。
在该研究中，发现碳酸钙和滑石粉分别将HVCP-Sm1的TGase活性从15 U/mL提高到29.05 U/mL和31 U/mL。此外，观察到碳酸钙和滑石粉会减小菌丝颗粒和伸长菌丝的直径，同时促进前TGase的激活。本研究扩展了对MPEC在丝状微生物形态及其对次级代谢影响方面作用的理解。
群体感应、能量代谢以及菌丝形态优化的协同作用增强了HVCP-Sm1的次级代谢并提高了TGase酶活性。
