Duc Luong Van, Nagao Shintaro, Mojarrad Mohammad, Miyagawa Yuta, Li Zi-Yan, Inoue Daisuke, Tajima Takahisa, Ike Michihiko
Division of Sustainable Energy and Environmental Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
Unit of Biotechnology, Graduate School of Integrated Sciences for Life, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8530, Japan.
Bioresour Technol. 2023 May;376:128853. doi: 10.1016/j.biortech.2023.128853. Epub 2023 Mar 9.
Ammonium (NH) and salinity (NaCl) inhibit CH production in anaerobic digestion. However, whether bioaugmentation using marine sediment-derived microbial consortia can relieve the inhibitory effects of NH and NaCl stresses on CH production remains unclear. Thus, this study evaluated the effectiveness of bioaugmentation using marine sediment-derived microbial consortia in alleviating the inhibition of CH production under NH or NaCl stress and elucidated the underlying mechanisms. Batch anaerobic digestion experiments under 5 gNH-N/L or 30 g/L NaCl were performed with or without augmentation using two marine sediment-derived microbial consortia pre-acclimated to high NH and NaCl. Compared with non-bioaugmentation, bioaugmentation reinforced CH production. Network analysis revealed the joint effects of microbial connections by Methanoculleus, which promoted the efficient consumption of propionate accumulated under NH and NaCl stresses. In conclusion, bioaugmentation with pre-acclimated marine sediment-derived microbial consortia can mitigate the inhibition under NH or NaCl stress and enhance CH production in anaerobic digestion.
铵（NH）和盐度（NaCl）会抑制厌氧消化过程中甲烷（CH）的产生。然而，利用海洋沉积物来源的微生物群落进行生物强化是否能够缓解NH和NaCl胁迫对CH产生的抑制作用仍不清楚。因此，本研究评估了利用海洋沉积物来源的微生物群落进行生物强化在缓解NH或NaCl胁迫下对CH产生的抑制作用的有效性，并阐明了其潜在机制。在5 gNH-N/L或30 g/L NaCl条件下进行批次厌氧消化实验，分别使用两个预先适应高NH和NaCl的海洋沉积物来源的微生物群落进行强化或不强化处理。与未进行生物强化相比，生物强化增强了CH的产生。网络分析揭示了甲烷袋状菌属（Methanoculleus）的微生物连接的联合作用，其促进了在NH和NaCl胁迫下积累的丙酸盐的有效消耗。总之，用预先适应的海洋沉积物来源的微生物群落进行生物强化可以减轻NH或NaCl胁迫下的抑制作用，并提高厌氧消化中CH的产生。
