Kabamba-Tshikongo A, Manya-Mboni H, Mwamba-Mulumba C, Takaisi-Kikuni N B, Vandenbroucke A T, Pâques A T, Dessilly G, Kabamba-Mukadi B, Longanga-Otshudi A
Laboratoire de Biologie Clinique, Faculté des Sciences Pharmaceutiques, Université de Lubumbashi, Lubumbashi, Democratic Republic of the Congo; Centre d'Excellence et d'Expertise des hépatites virales et autres pathologies, Lubumbashi, Democratic Republic of the Congo; Institut de Recherche Expérimentale et Clinique, Pôle de Microbiologie, Université Catholique de Louvain, Brussels, Belgium.
Laboratoire de Biologie Clinique, Faculté des Sciences Pharmaceutiques, Université de Lubumbashi, Lubumbashi, Democratic Republic of the Congo.
Transfus Clin Biol. 2023 May;30(2):272-278. doi: 10.1016/j.tracli.2023.02.007. Epub 2023 Mar 8.
To date, no study has been done yet on the distribution of Hepatitis C virus genotypes in Lubumbashi, Democratic Republic of Congo. The objective of this work was to determine the seroprevalence and study the distribution of hepatitis C virus (HCV) genotypes among blood donors in Lubumbashi, DRC.
This was a cross-sectional descriptive study among blood donors. The detection of anti-HCV antibodies was carried out by rapid diagnostic test (RDT) then confirmed by Chemiluminescent immuno-assay (CLIA). Viral load was determined by Nucleic Acid Amplification test (NAT) on Panther system and genotyping by Next Generation Sequencing (NGS) on Sentosa platform.
The obtained seroprevalence was 4.8%. Genotypes 3a (5.0%), 4 (90.0%) and 7 (5.0%) and a few drug resistance mutations were identified in the study population. Significant disturbances of some studied biochemical parameters (HDL-cholesterol, direct bilirubin, transaminases, ALP, GGT and albumin) have been observed in positive HCV blood donors. Irregular family and volunteer donors have been found as the socio-demographic characteristics associated with hepatitis C.
With a seroprevalence of 4.8% obtained among blood donors, Lubumbashi is in an area with medium endemicity for HCV, highlighting the need to implement strategies aiming to improve transfusion safety among blood recipients in Lubumbashi. This study reports for the first time the presence of HCV strains of genotypes 3a, 4 and 7. These results might allow better therapeutic management of HCV infections and contribute to the development of the mapping of HCV genotypes in Lubumbashi and DRC as well.
迄今为止，尚未对刚果民主共和国卢本巴希市丙型肝炎病毒基因型的分布进行过研究。本研究的目的是确定卢本巴希市献血者中丙型肝炎病毒（HCV）的血清流行率，并研究其基因型分布。
这是一项针对献血者的横断面描述性研究。通过快速诊断试验（RDT）检测抗HCV抗体，然后通过化学发光免疫分析（CLIA）进行确认。通过在Panther系统上进行核酸扩增试验（NAT）测定病毒载量，并在Sentosa平台上通过下一代测序（NGS）进行基因分型。
获得的血清流行率为4.8%。在研究人群中鉴定出3a型（5.0%）、4型（90.0%）和7型（5.0%）基因型以及一些耐药突变。在HCV阳性献血者中观察到一些研究的生化参数（高密度脂蛋白胆固醇、直接胆红素、转氨酶、碱性磷酸酶、γ-谷氨酰转移酶和白蛋白）有明显异常。发现非正规家庭献血者和志愿献血者是与丙型肝炎相关的社会人口学特征。
在献血者中获得的血清流行率为4.8%，卢本巴希属于HCV中度流行地区，这突出表明需要实施旨在提高卢本巴希市受血者输血安全性的策略。本研究首次报告了3a型、4型和7型HCV毒株的存在。这些结果可能有助于更好地治疗HCV感染，并有助于绘制卢本巴希市和刚果民主共和国的HCV基因型图谱。
