Laboratoire de biologie clinique, faculté des sciences pharmaceutiques, université de Lubumbashi, Lubumbashi, Democratic Republic of the Congo; Pôle de microbiologie, institut de recherche expérimentale et clinique, université catholique de Louvain, Brussels, Belgium.
Laboratoire de biologie clinique, faculté des sciences pharmaceutiques, université de Lubumbashi, Lubumbashi, Democratic Republic of the Congo.
Transfus Clin Biol. 2021 Feb;28(1):30-37. doi: 10.1016/j.tracli.2020.10.012. Epub 2020 Nov 21.
The strains of HBV circulating among blood donors in Lubumbashi, Democratic Republic of Congo (DRC), are not yet characterized. The purpose of this study was to determine seroprevalence, changes in biochemical parameters during HBV infection and molecular characterization of HBV in blood donors in Lubumbashi.
The detection of HBsAg was carried out by rapid diagnostic test then confirmed by the Liaison XL® Quant HBsAg technique. PCR targeting the P gene was carried out on LightCycler® 96 and genotyping by the sequencing technique on ABI 3500.
The seroprevalence was 7.9%. The genotypes E (53.1%), A (41.8%), A3/E (3.8%), A1/E (1.3%) and some drug resistance mutations were identified. Disturbances of HDL-cholesterol, direct bilirubin, transaminases (ASAT and ALAT), PAL, GGT and albumin have been observed in HBsAg positive blood donors.
The results of our study indicated that Lubumbashi is in a region with high endemicity for HBV and report for the first time HBV of genotypes A, E, A1/E and A3/E. They highlight the need to implement strategies to improve transfusion safety in blood transfusion centers and hospital blood banks in Lubumbashi in order to reduce HBV infection in recipients. They could also contribute to the implementation of treatment strategies and the development of mapping of circulating HBV genotypes in the DRC.
目前尚不清楚刚果民主共和国（DRC）卢本巴希献血者中流行的乙型肝炎病毒（HBV）株。本研究旨在确定卢本巴希献血者中 HBV 的血清流行率、HBV 感染期间生化参数的变化以及 HBV 的分子特征。
采用快速诊断试验检测 HBsAg，然后用 Liaison XL® Quant HBsAg 技术确认。在 LightCycler® 96 上进行针对 P 基因的 PCR，在 ABI 3500 上进行测序技术的基因分型。
血清流行率为 7.9%。鉴定出基因型 E（53.1%）、A（41.8%）、A3/E（3.8%）、A1/E（1.3%）和一些耐药突变。在 HBsAg 阳性献血者中观察到 HDL-胆固醇、直接胆红素、转氨酶（ASAT 和 ALAT）、PAL、GGT 和白蛋白的紊乱。
我们的研究结果表明，卢本巴希处于 HBV 高度流行地区，首次报告了基因型 A、E、A1/E 和 A3/E 的 HBV。它们强调需要实施策略，以改善卢本巴希输血中心和医院血库的输血安全性，以减少受血者的 HBV 感染。它们还可以为实施治疗策略和在刚果民主共和国绘制循环 HBV 基因型图做出贡献。
