PASTE：一种高通量的大片段 DNA 插入方法。

PASTE: a high-throughput method for large DNA insertions.

机构信息

Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Constituent College of Pakistan Institute of Engineering and Applied Sciences, Jhang Road, Faisalabad, Pakistan.

出版信息

Trends Plant Sci. 2023 May;28(5):509-511. doi: 10.1016/j.tplants.2023.02.013. Epub 2023 Mar 8.

DOI:10.1016/j.tplants.2023.02.013

PMID:36898908

Abstract

Prime editing (PE) enables precise genome editing at targeted locus without inducing double-stranded breaks (DSBs). Despite its precision, PE lacks the tendency to integrate large DNA fragments into the genome. Recently, Yarnall et al. reported clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 and an integrase-based system that conducts targeted integration of large DNA sequences (~36 kb) into the genome more efficiently.

摘要

Prime 编辑 (PE) 可在靶向基因座实现精确的基因组编辑，而不会诱导双链断裂 (DSB)。尽管其精确性，但 PE 缺乏将大片段 DNA 整合到基因组中的倾向。最近，Yarnall 等人报道了成簇规律间隔短回文重复序列 (CRISPR)/Cas9 和基于整合酶的系统，该系统可更有效地将大片段 DNA 序列（约 36 kb）靶向整合到基因组中。