Department of Medical Biotechnology, Dongguk University, Seoul, Republic of Korea.
Molecular Imaging and Neurovascular Research Laboratory, Department of Neurology, Dongguk University College of Medicine, Dongguk University Ilsan Hospital, Goyang, Republic of Korea.
Sci Rep. 2023 Mar 10;13(1):4043. doi: 10.1038/s41598-023-31122-9.
There is no blood biomarker diagnostic of arterial thrombosis. We investigated if arterial thrombosis per se was associated with alterations in complete blood count (CBC) and white blood cell (WBC) differential count in mice. Twelve-week-old C57Bl/6 mice were used for FeCl-mediated carotid thrombosis (n = 72), sham-operation (n = 79), or non-operation (n = 26). Monocyte count (/µL) at 30-min after thrombosis (median 160 [interquartile range 140-280]) was ~ 1.3-fold higher than at 30-min after sham-operation (120 [77.5-170]), and twofold higher than in non-operated mice (80 [47.5-92.5]). At day-1 and -4 post-thrombosis, compared with 30-min, monocyte count decreased by about 6% and 28% to 150 [100-200] and 115 [100-127.5], which however were about 2.1-fold and 1.9-fold higher than in sham-operated mice (70 [50-100] and 60 [30-75], respectively). Lymphocyte counts (/µL) at 1- and 4-days after thrombosis (mean ± SD; 3513 ± 912 and 2590 ± 860) were ~ 38% and ~ 54% lower than those in the sham-operated mice (5630 ± 1602 and 5596 ± 1437, respectively), and ~ 39% and ~ 55% lower than those in non-operated mice (5791 ± 1344). Post-thrombosis monocyte-lymphocyte-ratio (MLR) was substantially higher at all three time-points (0.050 ± 0.02, 0.046 ± 0.025, and 0.050 ± 0.02) vs. sham (0.003 ± 0.021, 0.013 ± 0.004, and 0.010 ± 0.004). MLR was 0.013 ± 0.005 in non-operated mice. This is the first report on acute arterial thrombosis-related alterations in CBC and WBC differential parameters.
目前尚无动脉血栓形成的血液生物标志物诊断方法。我们研究了动脉血栓形成本身是否与小鼠全血细胞计数（CBC）和白细胞分类计数的改变有关。使用 12 周龄的 C57Bl/6 小鼠进行 FeCl 介导的颈总动脉血栓形成（n=72）、假手术（n=79）或非手术（n=26）。血栓形成后 30 分钟的单核细胞计数（/µL）（中位数 160 [四分位距 140-280]）比假手术高约 1.3 倍（120 [77.5-170]），比未手术的小鼠高两倍（80 [47.5-92.5]）。与 30 分钟相比，血栓形成后第 1 天和第 4 天的单核细胞计数分别下降了约 6%和 28%，至 150 [100-200]和 115 [100-127.5]，但仍分别比假手术组高 2.1 倍和 1.9 倍（分别为 70 [50-100]和 60 [30-75]）。血栓形成后第 1 天和第 4 天的淋巴细胞计数（/µL）（均值±标准差；3513±912 和 2590±860）比假手术组低约 38%和 54%（分别为 5630±1602 和 5596±1437），比未手术组低约 39%和 55%（分别为 5791±1344）。血栓形成后，单核细胞-淋巴细胞比值（MLR）在所有三个时间点（0.050±0.02、0.046±0.025 和 0.050±0.02）均显著高于假手术组（0.003±0.021、0.013±0.004 和 0.010±0.004）。未手术组的 MLR 为 0.013±0.005。这是第一个关于急性动脉血栓形成相关全血细胞计数和白细胞分类参数改变的报告。
