Department of Pediatrics, Buddhist Tzu-Chi General Hospital, Hualien, Taiwan.
Pediatr Neonatol. 2011 Jun;52(3):155-60. doi: 10.1016/j.pedneo.2011.03.007. Epub 2011 May 6.
Cord blood banking has become more popular in recent years. Checking cord blood complete blood count (CBC) and white blood cell (WBC) differential counts (DCs) is essential before cryopreserving the cord blood units. Therefore, establishing the normal reference values of cord blood CBC and WBC DC is important in clinical practice and research.
To obtain a large-scale population-based normal CBC and WBC DC reference values of healthy neonates' cord blood from a public cord blood bank and to investigate the influence of the gender and delivery route.
From September 2001 to November 2006, the cord blood of healthy Taiwanese neonates with gestational age 36 weeks and more were collected by Tzu Chi Cord Blood Bank with written informed consents. All cord blood samples were analyzed by Sysmex XE2100 automated hematology analyzer (Sysmex Corporation, Kobe, Japan) to obtain the CBC. The WBC DC was calculated by manual method. We used Student's t test and Mann-Whitney U test for investigating the influences of gender and delivery route on the CBC and WBC DC reference values. The results were presented by mean±standard deviation or 2.5-97.5th percentiles.
In the study period, totally 5602 cord blood samples were collected eligibly for analysis. The cord blood CBC and WBC DC normal reference values were calculated. The female neonates had significantly higher mean corpuscular volume, platelet count, and WBC count, but lower red blood cell (RBC) count, hemoglobin (Hb), hematocrit, and mean corpuscular Hb concentration values (p<0.001). Newborns through vaginal delivery had significantly higher RBC count, Hb, hematocrit, platelet count, and WBC count (p<0.001). The percentages of some different types WBC were significantly influenced by gender and delivery routes. Male babies had higher lymphocyte, monocyte, eosinophil, basophil, and nucleated RBC ratios than the female neonates. Newborns through cesarean section had significantly lower neutrophil, monocyte, and nucleated RBC ratios, but higher lymphocyte and eosinophil ratios, than newborns through vaginal delivery.
We successfully obtained the normal CBC and WBC DC reference values of the cord blood in Taiwan. Gender and delivery routes were important confounding factors that influenced the cord blood CBC and WBC DC values.
近年来，脐带血库的应用越来越普及。在冷冻保存脐带血单位之前，检查脐带血的全血细胞计数（CBC）和白细胞（WBC）分类计数（DC）是至关重要的。因此，建立健康新生儿脐带血的大规模人群正常 CBC 和 WBC DC 参考值在临床实践和研究中非常重要。
从慈济脐带血库获取大量基于人群的健康台湾新生儿脐带血的正常 CBC 和 WBC DC 参考值，并研究性别和分娩方式的影响。
从 2001 年 9 月至 2006 年 11 月，以书面同意书的形式从 Tzu Chi Cord Blood Bank 收集胎龄 36 周及以上的健康台湾新生儿的脐带血。所有脐带血样本均由 Sysmex XE2100 自动血液分析仪（Sysmex 公司，神户，日本）分析以获得 CBC。通过手动方法计算 WBC DC。我们使用学生 t 检验和曼-惠特尼 U 检验来研究性别和分娩方式对 CBC 和 WBC DC 参考值的影响。结果以平均值±标准差或 2.5-97.5%分位数表示。
在研究期间，共有 5602 份脐带血样本符合分析条件。计算了脐带血 CBC 和 WBC DC 的正常参考值。女性新生儿的平均红细胞体积、血小板计数和白细胞计数显著较高，但红细胞计数、血红蛋白（Hb）、血细胞比容和平均红细胞 Hb 浓度值显著较低（p<0.001）。阴道分娩的新生儿的红细胞计数、Hb、血细胞比容、血小板计数和白细胞计数显著较高（p<0.001）。一些不同类型白细胞的百分比受到性别和分娩方式的显著影响。男婴的淋巴细胞、单核细胞、嗜酸性粒细胞、嗜碱性粒细胞和有核红细胞的比例高于女婴。剖宫产的新生儿中性粒细胞、单核细胞和有核红细胞的比例显著低于阴道分娩的新生儿，而淋巴细胞和嗜酸性粒细胞的比例显著高于阴道分娩的新生儿。
我们成功获得了台湾脐带血的正常 CBC 和 WBC DC 参考值。性别和分娩方式是影响脐带血 CBC 和 WBC DC 值的重要混杂因素。
