College of Agronomy, Hunan Agricultural University, Changsha, 410128, Hunan, People's Republic of China.
College of Horticulture, Shanxi Agricultural University, Taigu, 030801, Shanxi, People's Republic of China.
Sci Rep. 2023 Mar 10;13(1):4024. doi: 10.1038/s41598-023-31023-x.
In present study, sodium salt of α-naphthalene acetic acid (NA), potassium salt of fulvic acid (KF) and their combinations were applied to the growth substrates of tomato seedlings (Solanum lycopersicum L.) under chilling stress. The changes in aboveground biomass, root attributes, pigment contents, chlorophyll fluorescence, photosynthesis, osmotic regulation substances, and antioxidant enzymes activity of the tomato seedlings in response to NA and KF were investigated. The application of NA, KF and their combination could promote the growth of plant height and stem diameter of tomato seedlings under chilling stress to varying degrees, and improve root characteristics by increasing root volume, root length and root activity, and increase dry matter accumulation. In addition, the combined use of NA and KF improved the seedling leaf chlorophyll content, qP, Fv/Fm, ΦPSII , Pn and increased the activity of antioxidant enzymes in the tomato plants. The above results suggested a synergistic effect between NA and KF to stimulate the seedlings growth and to enhance the ROS scavenging ability of tomato, which has never been reported in previous research before. However, further researches are needed to explore the physiological and molecular mechanism underlying the synergistic effect between NA and KF.
在本研究中，应用α-萘乙酸钠盐（NA）、腐植酸钾（KF）及其组合处理番茄幼苗（Solanum lycopersicum L.）的生长基质，以应对冷胁迫。研究了 NA 和 KF 对番茄幼苗地上生物量、根系属性、色素含量、叶绿素荧光、光合作用、渗透调节物质和抗氧化酶活性的影响。结果表明，NA、KF 及其组合的应用能不同程度地促进冷胁迫下番茄幼苗株高和茎径的生长，通过增加根体积、根长和根活力来改善根系特性，并增加干物质积累。此外，NA 和 KF 的组合使用提高了幼苗叶片的叶绿素含量、qP、Fv/Fm、ΦPSII、Pn，并提高了番茄植株抗氧化酶的活性。上述结果表明，NA 和 KF 之间存在协同作用，能刺激幼苗生长，增强番茄的 ROS 清除能力，这在以前的研究中从未报道过。然而，需要进一步的研究来探索 NA 和 KF 之间协同作用的生理和分子机制。
