Dorofeeva A I, Shipunova I N, Luchkin A V, Abramova A V, Fidarova Z T, Dvirnyk V N, Galtseva I V, Mikhailova E A, Parovichnikova E N
National Medical Research Center of Hematology, Ministry of Health of the Russian Federation, Moscow, Russia.
Bull Exp Biol Med. 2023 Feb;174(4):538-543. doi: 10.1007/s10517-023-05744-y. Epub 2023 Mar 11.
The differentiation potential of individual clones of fibroblast CFU (CFU-F) was studied and the relative expression level of genes was analyzed in the culture of CFU-F from the bone marrow in patients with non-severe and severe forms of aplastic anemia at the onset of the disease. The differentiation potential of CFU-F clones was determined by the relative expression of marker genes using quantitative PCR. In aplastic anemia, the ratio of CFU-F clones with different differentiation potential changes, but the molecular mechanisms of this phenomenon are different in non-severe and severe aplastic anemia. In the culture of CFU-F in non-severe and severe aplastic anemia, the relative expression level of genes associated with the maintenance of the hematopoietic stem cell in the bone marrow niche changes, but the decrease in the expression of immunoregulatory genes occurs in severe form only, which may reflect differences in the pathogenesis of non-severe and severe aplastic anemia.
研究了成纤维细胞集落形成单位（CFU-F）单个克隆的分化潜能，并分析了非重型和重型再生障碍性贫血患者疾病初发时骨髓CFU-F培养物中基因的相对表达水平。通过定量PCR检测标记基因的相对表达来确定CFU-F克隆的分化潜能。在再生障碍性贫血中，具有不同分化潜能的CFU-F克隆比例发生变化，但这种现象的分子机制在非重型和重型再生障碍性贫血中有所不同。在非重型和重型再生障碍性贫血的CFU-F培养物中，与骨髓微环境中造血干细胞维持相关的基因相对表达水平发生变化，但免疫调节基因的表达仅在重型中降低，这可能反映了非重型和重型再生障碍性贫血发病机制的差异。
