Du Hongxu, Tan Xiaoyan, Li Zhangxun, Dong Hong, Su Lijuan, He Zhengke, Ma Qi, Dong Shiqi, Ramachandran Mythili, Liu Juan, Cao Liting
Department of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Southwest University, Chongqing 402460, China.
Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, Beijing University of Agriculture, Beijing 102206, China.
Animals (Basel). 2023 Mar 4;13(5):930. doi: 10.3390/ani13050930.
polysaccharide (SCP) is an experimental therapeutic for the treatment of intestinal injury. Selenium nanoparticle modification can improve the bioactivity of polysaccharides. In this study, SCP was firstly extracted and purified by a DEAE-52 column, then SCP-Selenium nanoparticles (SCP-Se NPs) were prepared, and the procedure was optimized. Thereafter, the obtained SCP-Se NPs were characterized by transmission electron microscope, X-ray diffraction, energy-dispersive X-ray spectroscopy, and Fourier transform infrared spectroscopy. The influence of different storage environments on the stability of colloidal SCP-Se NPs was also investigated. Finally, the therapeutic effects of SCP-Se NPs on LPS-induced intestinal inflammatory injuries in mice were evaluated. Results showed that the optimized SCP-Se NPs were amorphous, uniform, spherical particles with a diameter of 121 nm, and the colloidal solution was stable at 4 °C for at least 14 d. Moreover, SCP-Se NPs could more effectively alleviate LPS-induced diarrhea, intestinal tissue injury, and tight junction destruction and decrease the elevated expression levels of TNF-α, IL-1β, and IL-6 compared with SCP. These results demonstrate that SCP-Se NPs may alleviate LPS-induced enteritis through their anti-inflammatory effects, indicating that SCP-Se NPs can serve as a good candidate for preventing and treating enteritis in the livestock and poultry industry.
多糖（SCP）是一种用于治疗肠道损伤的实验性疗法。纳米硒修饰可以提高多糖的生物活性。在本研究中，首先通过DEAE - 52柱提取并纯化SCP，然后制备SCP - 硒纳米颗粒（SCP - Se NPs）并对其制备工艺进行优化。此后，通过透射电子显微镜、X射线衍射、能量色散X射线光谱和傅里叶变换红外光谱对所得的SCP - Se NPs进行表征。还研究了不同储存环境对胶体SCP - Se NPs稳定性的影响。最后，评估了SCP - Se NPs对脂多糖（LPS）诱导的小鼠肠道炎性损伤的治疗效果。结果表明，优化后的SCP - Se NPs为无定形、均匀的球形颗粒，直径为121 nm，其胶体溶液在4℃下至少14 d稳定。此外，与SCP相比，SCP - Se NPs能更有效地缓解LPS诱导的腹泻、肠道组织损伤和紧密连接破坏，并降低肿瘤坏死因子 - α（TNF - α）、白细胞介素 - 1β（IL - 1β）和白细胞介素 - 6（IL - 6）的升高表达水平。这些结果表明，SCP - Se NPs可能通过其抗炎作用减轻LPS诱导的肠炎，表明SCP - Se NPs可作为预防和治疗畜禽业肠炎的良好候选物。
