Department of Animal Sciences, College of Agriculture and Natural Resources, Razi University, Kermanshah, Iran.
Department of Plant Protection, College of Agriculture and Natural Resources, Razi University, Kermanshah, Iran.
Nucleosides Nucleotides Nucleic Acids. 2021;40(9):926-941. doi: 10.1080/15257770.2021.1964526. Epub 2021 Aug 14.
Nano-biotechnology has grown rapidly and become an integral part of modern disease diagnosis and treatment. The aim of this survey was to evaluate the anticancer activity of synthesized selenium nanoparticles (Se-NPs) against breast cancer cells (MCF-7). The prepared Se-NPs were examined by ultraviolet-visible spectroscopy, Fourier transform infrared (FTIR) spectroscopy, scanning electron microscope (SEM), and energy dispersive spectroscopy (EDX). Antioxidant activity of Se-NPs property was studied by radical scavenging (DPPH) assay. The in-vitro cytotoxicity of Se-NPs was evaluated by MTT assay. In addition; the biological assessment (antioxidant and cytotoxicity) of synthesized Se-NPs was examined via molecular docking simulations. Synthesis of Se-NPs was characterized by several studies such as UV-absorbance, showing peak values in the range of 268 nm. Nanoparticle sizes of the nanoparticles are confirmed by dynamic light scattering analysis, indicating that average size is about 203 nm. The quantity of selenium in Se-NPs is 90.15% by weight, as confirmed by EDX. Synthesized Se-NPs have anti-proliferative effects on MCF-7 cell lines. Cytotoxicity and apoptotic potential assays exhibited a dose-dependent effect against MCF-7 cells using an MTT assay. Like anti-cancer activity, anti-oxidant activity of Se-NPs was dose-dependent. Findings showed that the Se-NPs complexes have the highest inhibitory effect against cytotoxic and antioxidant receptors. Results of this study demonstrated that Se-NPs had strong potential to scavenge free radicals and are cytotoxic against the MCF-7 cancer cell line.
纳米生物技术发展迅速，已成为现代疾病诊断和治疗不可或缺的一部分。本研究旨在评估合成硒纳米粒子（Se-NPs）对乳腺癌细胞（MCF-7）的抗癌活性。通过紫外-可见光谱、傅里叶变换红外（FTIR）光谱、扫描电子显微镜（SEM）和能谱（EDX）对制备的 Se-NPs 进行了检测。通过自由基清除（DPPH）试验研究了 Se-NPs 的抗氧化活性。通过 MTT 试验评估了 Se-NPs 的体外细胞毒性。此外；通过分子对接模拟研究了合成 Se-NPs 的生物评估（抗氧化和细胞毒性）。通过几种研究对 Se-NPs 的合成进行了表征，例如 UV 吸收，在 268nm 范围内显示出峰值。通过动态光散射分析证实了纳米颗粒的粒径，表明平均粒径约为 203nm。通过 EDX 证实，Se-NPs 中的硒含量为 90.15%（重量）。合成的 Se-NPs 对 MCF-7 细胞系具有抗增殖作用。细胞毒性和凋亡潜力试验通过 MTT 试验显示对 MCF-7 细胞具有剂量依赖性效应。与抗癌活性一样，Se-NPs 的抗氧化活性也是剂量依赖性的。研究结果表明，Se-NPs 复合物对细胞毒性和抗氧化受体具有最高的抑制作用。本研究结果表明，Se-NPs 具有很强的清除自由基的潜力，并对 MCF-7 癌细胞系具有细胞毒性。
