Mijač Maja, Ljubin-Sternak Sunčanica, Ivković-Jureković Irena, Vraneš Jasmina
Molecular Microbiology Department, Dr. Andrija Štampar Teaching Institute of Public Health, 10000 Zagreb, Croatia.
Medical Microbiology Department, University of Zagreb School of Medicine, 10000 Zagreb, Croatia.
Diagnostics (Basel). 2023 Feb 23;13(5):846. doi: 10.3390/diagnostics13050846.
Human bocavirus (HBoV) is an important respiratory pathogen, especially in children, but it is often found in co-detection with other respiratory viruses, which makes the diagnostic approach challenging. We compared multiplex PCR and quantitative PCR for HBoV with multiplex tandem PCR (MT-PCR) in 55 cases of co-detection of HBoV and other respiratory viruses. In addition, we investigated whether there is a connection between the severity of the disease, measured by the localization of the infection, and amount of virus detected in the respiratory secretions. No statistically significant difference was found, but children with large amount of HBoV and other respiratory virus had a longer stay in hospital.
人博卡病毒（HBoV）是一种重要的呼吸道病原体，尤其在儿童中常见，但它常与其他呼吸道病毒共同检测到，这使得诊断方法具有挑战性。我们在55例HBoV与其他呼吸道病毒共同检测的病例中，比较了用于HBoV检测的多重PCR和定量PCR与多重串联PCR（MT-PCR）。此外，我们还研究了以感染部位衡量的疾病严重程度与呼吸道分泌物中检测到的病毒量之间是否存在关联。未发现统计学上的显著差异，但HBoV和其他呼吸道病毒载量高的儿童住院时间更长。
