School of Nutrition and Dietetics, University of Antioquia, Medellín 050010, Colombia.
Facultad de Ciencias Exactas y Aplicadas, Instituto Tecnológico Metropolitano (ITM), Institución Universitaria, Medellín 050034, Colombia.
Int J Environ Res Public Health. 2023 Feb 25;20(5):4165. doi: 10.3390/ijerph20054165.
Previous studies have indicated that mango fruit has a chemopreventive capacity against colorectal cancer cells. The objective of this research was to evaluate the effect of an aqueous extract of lyophilized mango pulp (LMPE) on colon adenocarcinoma cells (SW480) and their metastatic derivatives (SW620) death and cellular invasion. DNA fragmentation was assessed by TUNEL assay; autophagy and expression of DR4 and Bcl-2 by flow cytometry; the expression of 35 apoptosis-related proteins and of matrix metalloproteinases 7 and 9 by immunodetection; and the invasive capacity of the cells by Boyden chamber. The results showed that LMPE at 30 mg/mL and 48 h of exposure results in DNA fragmentation and apoptosis in SW480 ( < 0.001) and SW620 ( < 0.01) cells. Additionally, LMPE decreased autophagy in the SW480 and SW620 cell lines ( < 0.001), which could sensitize them to the DNA damage generated by LMPE. The LMPE did not modulate the expression of matrix metalloproteinases 7 and 9, nor did it affect cellular invasion processes in the SW480 and SW620 cell lines. In conclusion, LMPE induces apoptosis and decreases autophagy in SW480 and SW620 cells.
先前的研究表明，芒果果实具有预防结直肠癌细胞的化学预防能力。本研究的目的是评估冻干芒果果肉的水提物（LMPE）对结肠腺癌细胞（SW480）及其转移衍生物（SW620）的死亡和细胞侵袭的影响。通过 TUNEL 检测评估 DNA 片段化；通过流式细胞术评估自噬和 DR4 和 Bcl-2 的表达；通过免疫检测评估 35 种与凋亡相关的蛋白质和基质金属蛋白酶 7 和 9 的表达；通过 Boyden 室评估细胞的侵袭能力。结果表明，在 30 mg/mL 和 48 小时暴露于 LMPE 的情况下，SW480（<0.001）和 SW620（<0.01）细胞中的 DNA 片段化和凋亡。此外，LMPE 降低了 SW480 和 SW620 细胞系中的自噬（<0.001），这使它们对 LMPE 产生的 DNA 损伤敏感。LMPE 没有调节基质金属蛋白酶 7 和 9 的表达，也没有影响 SW480 和 SW620 细胞系中的细胞侵袭过程。总之，LMPE 可诱导 SW480 和 SW620 细胞发生凋亡并降低自噬。
