Laboratoire de Biochimie Médicale et Biologie Moléculaire, Université de Reims Champagne-Ardenne, 51097 Reims, France.
Matrice Extracellulaire et Dynamique Cellulaire-MEDyC, CNRS UMR 7369, 51097 Reims, France.
Int J Mol Sci. 2023 Feb 21;24(5):4291. doi: 10.3390/ijms24054291.
The expression of glypicans in different hair follicle (HF) compartments is still poorly understood. Heparan sulfate proteoglycans (HSPGs) distribution in HF is classically investigated by conventional histology, biochemical analysis, and immunohistochemistry. Our previous study proposed a novel approach to assess hair histology and glypican-1 (GPC1) distribution changes in the HF at different phases of the hair growth cycle using infrared spectral imaging (IRSI). We show in the present manuscript for the first time complementary data on the distribution of glypican-4 (GPC4) and glypican-6 (GPC6) in HF at different phases of the hair growth cycle using IR imaging. Findings were supported by Western blot assays focusing on the GPC4 and GPC6 expression in HFs. Like all proteoglycan features, the glypicans are characterized by a core protein to which sulfated and/or unsulfated glycosaminoglycan (GAG) chains are covalently linked. Our study demonstrates the capacity of IRSI to identify the different HF tissue structures and to highlight protein, proteoglycan (PG), GAG, and sulfated GAG distribution in these structures. The comparison between anagen, catagen, and telogen phases shows the qualitative and/or quantitative evolution of GAGs, as supported by Western blot. Thus, in one analysis, IRSI can simultaneously reveal the location of proteins, PGs, GAGs and sulfated GAGs in HFs in a chemical and label-free manner. From a dermatological point of view, IRSI may constitute a promising technique to study alopecia.
糖蛋白聚糖在不同毛囊 (HF) 隔室中的表达仍知之甚少。经典的研究方法是通过常规组织学、生化分析和免疫组织化学来研究肝素硫酸盐蛋白聚糖 (HSPGs) 在 HF 中的分布。我们之前的研究提出了一种新的方法，使用红外光谱成像 (IRSI) 评估 HF 在不同毛发生长周期阶段的毛发组织学和糖蛋白聚糖-1 (GPC1) 分布变化。在本文中，我们首次展示了使用 IR 成像在不同毛发生长周期阶段 HF 中糖蛋白聚糖-4 (GPC4) 和糖蛋白聚糖-6 (GPC6) 分布的补充数据。Western blot 检测结果支持了这一发现，该检测集中于 HF 中 GPC4 和 GPC6 的表达。与所有蛋白聚糖特征一样，糖蛋白聚糖的特征是核心蛋白，硫酸化和/或非硫酸化糖胺聚糖 (GAG) 链通过共价键与之相连。我们的研究表明 IRSI 能够识别不同的 HF 组织结构，并突出这些结构中蛋白质、蛋白聚糖 (PG)、GAG 和硫酸化 GAG 的分布。在一项分析中，IRSI 可以同时以化学和无标记的方式揭示 HF 中蛋白质、PGs、GAGs 和硫酸化 GAGs 的位置，这得到了 Western blot 的支持。从皮肤科的角度来看，IRSI 可能成为研究脱发的一种很有前途的技术。
