Department of Biochemistry and Biotechnology, Institute of Biological Sciences, Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland.
Department of Human Physiology, Medical University of Lublin, 11 Radziwiłowska Street, 20-080 Lublin, Poland.
Int J Mol Sci. 2023 Feb 25;24(5):4535. doi: 10.3390/ijms24054535.
Cellobiose dehydrogenase (CDH) is an extracellular hemoflavoprotein catalyzing the oxidation reaction of β-1,4-glycosidic-bonded sugars (lactose or cellobiose), which results in the formation of aldobionic acids and hydrogen peroxide as a byproduct. The biotechnological application of CDH requires the immobilization of the enzyme on a suitable support. As a carrier of natural origin used for CDH immobilization, chitosan seems to increase the catalytic potential of the enzyme, especially for applications as packaging in the food industry and as a dressing material in medical applications. The present study aimed to immobilize the enzyme on chitosan beads and determine the physicochemical and biological properties of immobilized CDHs obtained from different fungal sources. The chitosan beads with immobilized CDHs were characterized in terms of their FTIR spectra or SEM microstructure. The most effective method of immobilization in the proposed modification was the covalent bonding of enzyme molecules using glutaraldehyde, resulting in efficiencies ranging from 28 to 99%. Very promising results, compared to free CDH, were obtained in the case of antioxidant, antimicrobial, and cytotoxic properties. Summarizing the obtained data, chitosan seems to be a valuable material for the development of innovative and effective immobilization systems for biomedical applications or food packaging, preserving the unique properties of CDH.
纤维二糖脱氢酶（CDH）是一种细胞外血红素蛋白，能够催化β-1,4-糖苷键结合的糖（乳糖或纤维二糖）的氧化反应，生成醛糖酸和过氧化氢作为副产物。CDH 的生物技术应用需要将酶固定在合适的载体上。壳聚糖作为一种天然来源的载体，用于 CDH 的固定化，似乎可以提高酶的催化潜力，特别是在食品工业中的包装应用和医疗应用中的敷料材料方面。本研究旨在将酶固定在壳聚糖珠上，并确定来自不同真菌来源的固定化 CDH 的物理化学和生物学性质。通过傅里叶变换红外光谱（FTIR）或扫描电子显微镜（SEM）微结构对固定化 CDH 的壳聚糖珠进行了表征。在提出的修饰中，最有效的固定化方法是使用戊二醛共价键合酶分子，效率范围为 28%至 99%。与游离 CDH 相比，在抗氧化、抗菌和细胞毒性方面，固定化 CDH 获得了非常有前景的结果。综上所述，壳聚糖似乎是一种有价值的材料，可用于开发用于生物医学应用或食品包装的创新且有效的固定化系统，保持 CDH 的独特性质。
