Morris G S, Simmonds H A, Toseland P A, Van Acker K J, Davies P M, Stuchbury J H
Purine Laboratory, United Medical School of Guy's Hospital, London.
Br J Urol. 1987 Oct;60(4):292-300. doi: 10.1111/j.1464-410x.1987.tb04971.x.
We showed previously that ingestion of a non-specific high purine diet by healthy subjects increased not only urinary uric acid levels but urinary oxalate as well. Both increments were reduced significantly during concomitant allopurinol therapy. The present study was undertaken to investigate these findings in more detail under carefully controlled dietary conditions where a single specific purine, guanosine, was used as an additive and several different methods for oxalate determination (GLC, HPLC, isotacophoresis) were compared with the enzymatic method used previously. Results obtained by two direct techniques of oxalate determination showed no significant alteration in oxalate levels during any dietary regime, suggesting that the earlier results derived from problems inherent in the experimental design and methodology employed. The study confirmed that one of the beneficial effects of allopurinol was to reduce dietary purine absorption. The results may thus provide a logical explanation for the reduced incidence of urolithiasis during allopurinol therapy in some idiopathic oxalate stone formers addicted to purine-rich foods and beverages.
我们之前的研究表明，健康受试者摄入非特异性高嘌呤饮食不仅会增加尿尿酸水平，还会增加尿草酸水平。在同时进行别嘌醇治疗期间，这两种增加都显著降低。本研究旨在在严格控制的饮食条件下更详细地研究这些发现，其中使用单一特定嘌呤鸟苷作为添加剂，并将几种不同的草酸测定方法（气相色谱法、高效液相色谱法、等速电泳法）与之前使用的酶法进行比较。通过两种直接草酸测定技术获得的结果表明，在任何饮食方案下草酸水平均无显著变化，这表明早期结果源于所采用的实验设计和方法中固有的问题。该研究证实，别嘌醇的有益作用之一是减少饮食中嘌呤的吸收。因此，这些结果可能为某些沉迷于富含嘌呤的食物和饮料的特发性草酸结石形成者在别嘌醇治疗期间尿石症发病率降低提供一个合理的解释。
