Clarke D, Ramaswamy A, Holmes L, Mudd L, Poulakos J, Raizada M K
Department of Pediatrics, University of Florida College of Medicine, Gainesville 32610.
Brain Res. 1987 Sep 22;421(1-2):358-62. doi: 10.1016/0006-8993(87)91307-2.
Activation of protein kinase C by phorbol esters caused a time- and dose-dependent stimulation (270% of control) of glucose uptake in cultured glia, but not in neurons from rat brain. The phorbol ester stimulation of Vmax of glial glucose uptake occurred only in glia despite nearly 2.5-fold greater phorbol ester binding in neurons. These differences in cellular responses to protein kinase C activation may be the key to understanding brain glucose regulation.
佛波酯激活蛋白激酶C可引起培养的神经胶质细胞葡萄糖摄取呈时间和剂量依赖性刺激（为对照的270%），但对大鼠脑神经元无此作用。尽管神经元中佛波酯结合量几乎是神经胶质细胞的2.5倍，但佛波酯对神经胶质细胞葡萄糖摄取Vmax的刺激仅在神经胶质细胞中出现。细胞对蛋白激酶C激活的这些反应差异可能是理解脑葡萄糖调节的关键。
