Hanani M, Lin Z, Louzon V, Brenner T, Boneh A
Laboratory of Experimental Surgery, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Neurosci Lett. 1997 Sep 19;233(2-3):61-4. doi: 10.1016/s0304-3940(97)00630-7.
Cultures of myenteric ganglia from adult guinea-pigs were used to study the influence of neuroactive substances on glial cells by monitoring changes in their morphology. The following substances had no effect on glial morphology: adenosine, ATP, carbachol, glutamate, bradykinin, isoprenaline, prostaglandin E2, sodium nitroprusside and lipopolysaccharide. The only substances found to affect glial morphology were phorbol esters, and in particular phorbol 12-myrisate 13-acetate (PMA), which acted at the nM range. Glial cells, which were normally polygonal, assumed a stellate shape within 30-60 min after the addition of PMA. Protein kinase C (PKC) inhibitors did not block this effect, and PKC activators did not mimic it. The effect of PMA was also not mediated by changes in the intracellular concentrations of either Ca2+, H+ or cyclic AMP. Dye coupling among glial cells was blocked by PMA. The phorbol ester-mediated effect on glial structure may have profound influence on neuronal organization and function.
利用成年豚鼠的肠肌间神经节培养物，通过监测神经活性物质对神经胶质细胞形态的影响来进行研究。以下物质对神经胶质细胞形态没有影响：腺苷、三磷酸腺苷、卡巴胆碱、谷氨酸、缓激肽、异丙肾上腺素、前列腺素E2、硝普钠和脂多糖。唯一被发现影响神经胶质细胞形态的物质是佛波酯，特别是佛波醇12 - 肉豆蔻酸酯13 - 乙酸酯（PMA），其作用浓度在纳摩尔范围内。通常呈多边形的神经胶质细胞在添加PMA后30 - 60分钟内会变成星状。蛋白激酶C（PKC）抑制剂不能阻断这种作用，PKC激活剂也不能模拟这种作用。PMA的作用也不是由细胞内钙离子、氢离子或环磷酸腺苷浓度的变化介导的。神经胶质细胞之间的染料偶联被PMA阻断。佛波酯介导的对神经胶质细胞结构的作用可能对神经元的组织和功能有深远影响。
