Huang Jiapeng, Shi Jinyuan, Wu Pu, Sun Wei, Zhang Dalin, Wang Zhihong, Ji Xiaoyu, Lv Chengzhou, Zhang Ting, Zhang Ping, Zhang Hao
Department of Thyroid Surgery, The First Hospital of China Medical University, Shenyang 110000, China.
J Clin Med. 2023 Mar 3;12(5):2014. doi: 10.3390/jcm12052014.
Cuproptosis is a novel programmed cell death that depends on copper. The role and potential mechanism of cuproptosis-related genes (CRGs) in thyroid cancer (THCA) are still unclear. In our study, we randomly divided THCA patients from the TCGA database into a training set and a testing set. A cuproptosis-related signature consisting of six genes (SLC31A1, LIAS, DLD, MTF1, CDKN2A, and GCSH) was constructed using the training set to predict the prognosis of THCA and was verified with the testing set. All patients were classified into low- and high-risk groups according to risk score. Patients in the high-risk group had a poorer overall survival (OS) than those in the low-risk group. The area under the curve (AUC) values for 5 years, 8 years, and 10 years were 0.845, 0.885, and 0.898, respectively. The tumor immune cell infiltration and immune status were significantly higher in the low-risk group, which indicated a better response to immune checkpoint inhibitors (ICIs). The expression of six cuproptosis-related genes in our prognostic signature were verified by qRT-PCR in our THCA tissues, and the results were consistent with TCGA database. In summary, our cuproptosis-related risk signature has a good predictive ability regarding the prognosis of THCA patients. Targeting cuproptosis may be a better alternative for THCA patients.
铜死亡是一种依赖于铜的新型程序性细胞死亡。铜死亡相关基因（CRGs）在甲状腺癌（THCA）中的作用和潜在机制仍不清楚。在我们的研究中，我们将来自TCGA数据库的THCA患者随机分为训练集和测试集。使用训练集构建了一个由六个基因（SLC31A1、LIAS、DLD、MTF1、CDKN2A和GCSH）组成的铜死亡相关特征，以预测THCA的预后，并在测试集中进行了验证。根据风险评分将所有患者分为低风险组和高风险组。高风险组患者的总生存期（OS）比低风险组患者差。5年、8年和10年的曲线下面积（AUC）值分别为0.845、0.885和0.898。低风险组的肿瘤免疫细胞浸润和免疫状态显著更高，这表明对免疫检查点抑制剂（ICIs）的反应更好。我们通过qRT-PCR在我们的THCA组织中验证了我们预后特征中六个铜死亡相关基因的表达，结果与TCGA数据库一致。总之，我们的铜死亡相关风险特征对THCA患者的预后具有良好的预测能力。针对铜死亡可能是THCA患者更好的选择。
