Malinowska Katarzyna, Kowalski Andrzej, Merecz-Sadowska Anna, Paprocka-Zjawiona Milena, Sitarek Przemysław, Kowalczyk Tomasz, Zielińska-Bliźniewska Hanna
Department of Allergology and Respiratory Rehabilitation, Medical University of Lodz, 90-725 Lodz, Poland.
Department of Otolaryngology, Laryngological Oncology, Audiology and Phoniatrics, Medical University of Lodz, 90-549 Lodz, Poland.
J Clin Med. 2023 Mar 3;12(5):2033. doi: 10.3390/jcm12052033.
Inflammation is an etiological factor of various chronic diseases contributing to more than 50% of worldwide deaths. In this study, we focus on the immunosuppressive role of the programmed death-1 (PD-1) receptor and its ligand (PD-L1) in inflammatory-related diseases, including chronic rhinosinusitis and head and neck cancers. The study included 304 participants. Of this number, 162 patients had chronic rhinosinusitis with nasal polyps (CRSwNP), 40 patients had head and neck cancer (HNC) and there were 102 healthy subjects. The expression level of the and genes in the tissues of the study groups was measured by qPCR and Western blot methods. The associations between the age of the patients and the extent of disease and genes' expression were evaluated. The study showed a significantly higher mRNA expression of and in the tissues of both the CRSwNP and HNC patient groups compared to the healthy group. The severity of CRSwNP significantly correlated with the mRNA expression of and . Similarly, the age of the NHC patients influenced expression. In addition, a significantly higher level of PD-L1 protein was noticed also for both the CRSwNP and HNC patient groups. The increased expression of and may be a potential biomarker of inflammatory-related diseases, including chronic rhinosinusitis and head and neck cancers.
炎症是多种慢性疾病的病因，全球超过50%的死亡与之相关。在本研究中，我们聚焦于程序性死亡-1（PD-1）受体及其配体（PD-L1）在炎症相关疾病中的免疫抑制作用，这些疾病包括慢性鼻-鼻窦炎和头颈部癌症。该研究纳入了304名参与者。其中，162例患者患有伴鼻息肉的慢性鼻-鼻窦炎（CRSwNP），40例患者患有头颈部癌症（HNC），还有102名健康受试者。通过qPCR和蛋白质印迹法检测研究组组织中 和 基因的表达水平。评估患者年龄与疾病程度及基因表达之间的关联。研究表明，与健康组相比，CRSwNP和HNC患者组组织中 和 的mRNA表达均显著更高。CRSwNP的严重程度与 和 的mRNA表达显著相关。同样，NHC患者的年龄影响 表达。此外，CRSwNP和HNC患者组的PD-L1蛋白水平也均显著更高。 和 表达的增加可能是包括慢性鼻-鼻窦炎和头颈部癌症在内的炎症相关疾病的潜在生物标志物。
