Canepa Paolo, Canale Claudio, Cavalleri Ornella, Marletta Giovanni, Messina Grazia M L, Messori Massimo, Novelli Rubina, Mattioli Simone Luca, Apparente Lucia, Detta Nicola, Romeo Tiziana, Allegretti Marcello
Dipartimento di Fisica, Università di Genova, Via Dodecaneso 33, 16146 Genova, Italy.
Laboratory for Molecular Surface and Nanotechnology (LAMSUN), Dipartimento di Scienze Chimiche, Università di Catania and CSGI, Viale A. Doria 6, 95125 Catania, Italy.
Materials (Basel). 2023 Mar 3;16(5):2076. doi: 10.3390/ma16052076.
The surface properties of drug containers should reduce the adsorption of the drug and avoid packaging surface/drug interactions, especially in the case of biologically-derived products. Here, we developed a multi-technique approach that combined Differential Scanning Calorimetry (DSC), Atomic Force Microscopy (AFM), Contact Angle (CA), Quartz Crystal Microbalance with Dissipation monitoring (QCM-D), and X-ray Photoemission Spectroscopy (XPS) to investigate the interactions of rhNGF on different pharma grade polymeric materials. Polypropylene (PP)/polyethylene (PE) copolymers and PP homopolymers, both as spin-coated films and injected molded samples, were evaluated for their degree of crystallinity and adsorption of protein. Our analyses showed that copolymers are characterized by a lower degree of crystallinity and lower roughness compared to PP homopolymers. In line with this, PP/PE copolymers also show higher contact angle values, indicating a lower surface wettability for the rhNGF solution on copolymers than PP homopolymers. Thus, we demonstrated that the chemical composition of the polymeric material and, in turn, its surface roughness determine the interaction with the protein and identified that copolymers may offer an advantage in terms of protein interaction/adsorption. The combined QCM-D and XPS data indicated that protein adsorption is a self-limiting process that passivates the surface after the deposition of roughly one molecular layer, preventing any further protein adsorption in the long term.
药物容器的表面性质应减少药物的吸附，并避免包装表面与药物之间的相互作用，尤其是对于生物衍生产品而言。在此，我们开发了一种多技术方法，该方法结合了差示扫描量热法（DSC）、原子力显微镜（AFM）、接触角（CA）、带耗散监测的石英晶体微天平（QCM-D）和X射线光电子能谱（XPS），以研究重组人神经生长因子（rhNGF）在不同药用级聚合物材料上的相互作用。对聚丙烯（PP）/聚乙烯（PE）共聚物和PP均聚物，以旋涂膜和注塑样品的形式，评估了它们的结晶度和蛋白质吸附程度。我们的分析表明，与PP均聚物相比，共聚物的结晶度较低且粗糙度较小。与此一致的是，PP/PE共聚物的接触角值也更高，这表明rhNGF溶液在共聚物上的表面润湿性低于PP均聚物。因此，我们证明了聚合物材料的化学成分，进而其表面粗糙度决定了与蛋白质的相互作用，并确定共聚物在蛋白质相互作用/吸附方面可能具有优势。QCM-D和XPS的综合数据表明，蛋白质吸附是一个自限性过程，在沉积大约一个分子层后使表面钝化，从长远来看可防止任何进一步的蛋白质吸附。
