Institute of Biophysics, Biological Research Centre Szeged, H-6726 Szeged, Hungary.
Department of Biotechnology, University of Szeged, H-6726 Szeged, Hungary.
Molecules. 2023 Feb 28;28(5):2261. doi: 10.3390/molecules28052261.
Cytochrome 561 proteins (CYB561s) are integral membrane proteins with six trans-membrane domains, two heme- redox centers, one on each side of the host membrane. The major characteristics of these proteins are their ascorbate reducibility and trans-membrane electron transferring capability. More than one CYB561 can be found in a wide range of animal and plant phyla and they are localized in membranes different from the membranes participating in bioenergization. Two homologous proteins, both in humans and rodents, are thought to participate-via yet unidentified way-in cancer pathology. The recombinant forms of the human tumor suppressor 101F6 protein (Hs_CYB561D2) and its mouse ortholog (Mm_CYB561D2) have already been studied in some detail. However, nothing has yet been published about the physical-chemical properties of their homologues (Hs_CYB561D1 in humans and Mm_CYB561D1 in mice). In this paper we present optical, redox and structural properties of the recombinant Mm_CYB561D1, obtained based on various spectroscopic methods and homology modeling. The results are discussed in comparison to similar properties of the other members of the CYB561 protein family.
细胞色素 561 蛋白（CYB561s）是具有六个跨膜结构域的完整膜蛋白，两个血红素-氧化还原中心，分别位于宿主膜的两侧。这些蛋白的主要特征是它们的抗坏血酸还原能力和跨膜电子传递能力。在广泛的动物和植物门中都可以发现一种以上的 CYB561，它们定位于与生物能量学无关的膜上。两种同源蛋白，无论是在人类还是啮齿动物中，都被认为以尚未确定的方式参与癌症病理学。人类肿瘤抑制因子 101F6 蛋白（Hs_CYB561D2）及其小鼠同源物（Mm_CYB561D2）的重组形式已经进行了一些详细的研究。然而，关于其同源物（人类中的 Hs_CYB561D1 和小鼠中的 Mm_CYB561D1）的物理化学性质尚未有任何报道。在本文中，我们根据各种光谱方法和同源建模，介绍了重组 Mm_CYB561D1 的光学、氧化还原和结构性质。并将结果与 CYB561 蛋白家族的其他成员的相似性质进行了讨论。
