Yang Q Z, Hatton G I
Neuroscience Program, Michigan State University, East Lansing 48824-1117.
Brain Res Bull. 1987 Nov;19(5):559-65. doi: 10.1016/0361-9230(87)90073-6.
To assess the possibility that dye coupling among neurons in hypothalamic slices might require dendrotomy, as has been suggested for neocortical neurons, dye coupling was studied in horizontally cut slices containing the supraoptic nucleus (SON). Since the dendrites of SON neurons project toward the pial surface, dendritic damage due to slicing can be avoided in the horizontal plane. Intracellular injections of Lucifer Yellow into individual SON neurons in slices from male, virgin female and lactating, mother rats yielded the following results. When dendrotomy occurred there was a significantly lower incidence of dye coupling than was observed when dendrites were intact. Higher order coupling (3 or more cells dye coupled after a single injection) was only seen among neurons without dendrotomy. Independently of dendritic damage, incidence of dye coupling in nursing mothers was reliably greater than for virgins, confirming previous results from coronal slices. The results of this study indicate that dendrotomy is not an inducer of dye coupling in SON neurons. Taken together with other recent findings, these data suggest that a reinterpretation of the effects of dendrotomy on cortical cell dye coupling may be in order.
为了评估下丘脑切片中神经元之间的染料偶联是否可能像对新皮层神经元所建议的那样需要进行树突切断术，我们在包含视上核（SON）的水平切片中研究了染料偶联。由于SON神经元的树突向软脑膜表面投射，因此在水平平面上可以避免切片导致的树突损伤。对来自雄性、未生育雌性和哺乳期母鼠的切片中的单个SON神经元进行细胞内注射荧光黄，得到以下结果。当发生树突切断时，染料偶联的发生率明显低于树突完整时观察到的发生率。高阶偶联（单次注射后3个或更多细胞发生染料偶联）仅在没有树突切断的神经元中出现。与树突损伤无关，哺乳期母鼠中染料偶联的发生率确实高于未生育母鼠，这证实了先前冠状切片的结果。本研究结果表明，树突切断术不是SON神经元中染料偶联的诱导因素。结合其他近期发现，这些数据表明可能需要重新解释树突切断术对皮层细胞染料偶联的影响。
