用于紧凑型双电极电压钳制系统的集成微电极的卵母细胞阵列流控装置。

Oocyte Array Fluidic Device Integrated with Microelectrodes for A Compact Two-Electrode Voltage Clamping System.

机构信息

School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Chuo-ku, Sagamihara 252-5201, Kanagawa, Japan.

Department of Electrical and Electronic Information Engineering, Toyohashi University of Technology, 1-1 Hibarigaoka, Tempaku-cho, Toyohashi 441-8580, Aichi, Japan.

出版信息

Sensors (Basel). 2023 Feb 21;23(5):2370. doi: 10.3390/s23052370.

DOI:10.3390/s23052370

PMID:36904573

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10007382/

Abstract

We report on a compact two-electrode voltage clamping system composed of microfabricated electrodes and a fluidic device for oocytes. The device was fabricated by assembling Si-based electrode chips and acrylic frames to form fluidic channels. After the installation of oocytes into the fluidic channels, the device can be separated in order to measure changes in oocyte plasma membrane potential in each channel using an external amplifier. Using fluid simulations and experiments, we investigated the success rates of oocyte arrays and electrode insertion with respect to the flow rate. We successfully located each oocyte in the array and detected oocyte responses to chemical stimuli using our device.

摘要

我们报告了一种由微加工电极和用于卵母细胞的流体装置组成的紧凑型双电极电压钳系统。该装置通过组装基于 Si 的电极芯片和丙烯酸框架来形成流体通道来制造。在将卵母细胞安装到流体通道中之后，可以将其分离，以便使用外部放大器测量每个通道中卵母细胞质膜电位的变化。我们使用流体模拟和实验研究了流速对卵母细胞阵列和电极插入的成功率的影响。我们成功地将每个卵母细胞定位在阵列中，并使用我们的装置检测卵母细胞对化学刺激的反应。