Bio Microsystems Project, Kanagawa Academy of Science and Technology (KAST), Kawasaki City, Japan.
J Am Chem Soc. 2011 Jun 8;133(22):8474-7. doi: 10.1021/ja2026085. Epub 2011 May 17.
This paper describes a methodology for the rapid and highly selective detection of cocaine using a membrane protein channel combined with a DNA aptamer. The DNA aptamer recognizes the cocaine molecule with high selectivity. We successfully detected a low concentration of cocaine (300 ng/mL, the drug test cutoff limit) within 60 s using a biological nanopore embedded in a microchip.
本文描述了一种使用膜蛋白通道结合 DNA 适体快速、高选择性检测可卡因的方法。该 DNA 适体对可卡因分子具有高选择性。我们成功地在微芯片中嵌入的生物纳米孔内，在 60 秒内检测到低浓度的可卡因（300ng/ml，药物检测截止值）。
