Plant Protection Department, College of Food and Agriculture Sciences, King Saud University, Saudi Arabia.
Department of Plant Production, College of Food and Agriculture Science, King Saud University, Riyadh, Saudi Arabia.
Cell Mol Biol (Noisy-le-grand). 2022 Sep 30;68(9):129-134. doi: 10.14715/cmb/2022.68.9.20.
Begomovirus is the largest genus in the family Geminiviridae and constitutes more than 445 virus species. Begomoviruses are characterized by single-stranded circular genomes with monopartite or bipartite components and transmitted by whitefly (Bemisia tabaci). Begomoviruses cause severe diseases in many economically important crops throughout the world. Typical symptoms of a begomovirus infection including severe leaf curling, vein thickening, vein darkening and reduced leaf size were observed in papaya plants in the Dammam district of the Eastern Province of Saudi Arabia during the growing season in 2022. A total of 10 samples were collected, and total genomic DNA was isolated from naturally infected papaya tree samples and subjected to PCR amplification using universal diagnostic primers for begomoviruses and associated satellites. Three PCR-amplified genomic components of begomoviruses and betasatellite namely P61Begomo (645 bp), P62Begomo (341 bp) and P62Beta (563 bp) were sent for Sanger DNA sequencing to Macrogen Inc. These partial viral genome sequences were submitted to Genbank database and accession numbers ON206051, ON206052 and ON206050 were assigned to P61Begomo, P62Begomo and P62Beta respectively. Phylogenetic analysis and pairwise nucleotide sequence identity studies identified P61Begomo was identified as Tomato yellow leaf curl virus, P62Begomo as DNA A component of a bipartite begomovirus Watermelon chlorotic stunt virus and P62Beta as begomovirus associated betasatellite; Cotton leaf curl Gezira betasatellite. To the best of our knowledge, this is the first report of a begomovirus complex infecting papaya (Carica papaya) in the Kingdom of Saudi Arabia.
双生病毒是双生病毒科中最大的属，由超过 445 种病毒组成。双生病毒的特征是单链环状基因组，具有单分体或二分体成分，并通过粉虱（烟粉虱）传播。双生病毒在全球许多经济重要作物中引起严重疾病。2022 年生长季节，在沙特阿拉伯东部省达曼地区的木瓜植株上观察到双生病毒感染的典型症状，包括严重的叶片卷曲、叶脉增厚、叶脉变暗和叶片变小。共采集了 10 个样本，从自然感染的木瓜树样本中提取总基因组 DNA，并使用双生病毒和相关卫星的通用诊断引物进行 PCR 扩增。使用 Sanger DNA 测序将三个双生病毒和 betasatellite 的 PCR 扩增基因组组件，即 P61Begomo（645 bp）、P62Begomo（341 bp）和 P62Beta（563 bp）发送给 Macrogen Inc. 这些部分病毒基因组序列被提交到 Genbank 数据库，并分别分配 ON206051、ON206052 和 ON206050 号登录号。系统进化分析和成对核苷酸序列同一性研究表明，P61Begomo 被鉴定为番茄黄卷叶病毒，P62Begomo 为双分体双生病毒西瓜褪绿斑驳病毒的 DNA A 成分，P62Beta 为双生病毒相关 betasatellite；棉花曲叶 Gezira betasatellite。据我们所知，这是首次在沙特阿拉伯报道双生病毒复合感染木瓜（Carica papaya）。
